GRIK2 Protein, Human (HEK293, hFc)

GRIK2 is an ionotropic glutamate receptor that plays an important role in central nervous system synapses, responding to L-glutamate as an excitatory neurotransmitter. Upon binding of L-glutamate, GRIK2 undergoes a conformational change that opens cation channels that convert chemical signals into electrical impulses. GRIK2 Protein, Human (HEK293, hFc) is the recombinant human-derived GRIK2 protein, expressed by HEK293 , with C-hFc labeled tag.

GRIK2, an ionotropic glutamate receptor, serves as a pivotal component in central nervous system synapses where L-glutamate acts as an excitatory neurotransmitter. Upon binding of L-glutamate, the receptor undergoes a conformational change, facilitating the opening of the cation channel and translating the chemical signal into an electrical impulse. Subsequently, the receptor swiftly desensitizes and enters a transient inactive state marked by the presence of bound agonist. Beyond its ionotropic glutamate receptor activity, GRIK2 plays a role in modulating the cell surface expression of NETO2 and functions as a thermoreceptor, conferring sensitivity to cold temperatures, particularly in dorsal root ganglion neurons.

Measured by its ability to inhibit cell migration of AGS cells.

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  Measured by its ability to inhibit cell migration of AGS cells.

Human

HEK293

C-hFc

Q13002/NP_001159719.1 (T32-P561)

2898  [NCBI]

Extracellular Domain

TTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSP

Approximately 100-115 kDa due to the glycosylation

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.