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  3. GRIK2 Protein, Human (HEK293, hFc)

GRIK2 Protein, Human (HEK293, hFc)

Cat. No.: HY-P75156
COA Handling Instructions

GRIK2 is an ionotropic glutamate receptor that plays an important role in central nervous system synapses, responding to L-glutamate as an excitatory neurotransmitter. Upon binding of L-glutamate, GRIK2 undergoes a conformational change that opens cation channels that convert chemical signals into electrical impulses. GRIK2 Protein, Human (HEK293, hFc) is the recombinant human-derived GRIK2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GRIK2 Protein, Human (HEK293, hFc) is 530 a.a., with molecular weight of ~100-115 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

GRIK2 is an ionotropic glutamate receptor that plays an important role in central nervous system synapses, responding to L-glutamate as an excitatory neurotransmitter. Upon binding of L-glutamate, GRIK2 undergoes a conformational change that opens cation channels that convert chemical signals into electrical impulses. GRIK2 Protein, Human (HEK293, hFc) is the recombinant human-derived GRIK2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GRIK2 Protein, Human (HEK293, hFc) is 530 a.a., with molecular weight of ~100-115 kDa.

Background

GRIK2, an ionotropic glutamate receptor, serves as a pivotal component in central nervous system synapses where L-glutamate acts as an excitatory neurotransmitter. Upon binding of L-glutamate, the receptor undergoes a conformational change, facilitating the opening of the cation channel and translating the chemical signal into an electrical impulse. Subsequently, the receptor swiftly desensitizes and enters a transient inactive state marked by the presence of bound agonist. Beyond its ionotropic glutamate receptor activity, GRIK2 plays a role in modulating the cell surface expression of NETO2 and functions as a thermoreceptor, conferring sensitivity to cold temperatures, particularly in dorsal root ganglion neurons.

Biological Activity

Measured by its ability to inhibit cell migration of AGS cells.

  • Measured by its ability to inhibit cell migration of AGS cells.
Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q13002/NP_001159719.1 (T32-P561)

Gene ID
Molecular Construction
N-term
GRIK2 (T32-P561)
Accession # NP_001159719.1/Q13002
hFc
C-term
Synonyms
Glutamate receptor ionotropic, kainate 2; GluK2; EAA4; GluR6
AA Sequence

TTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSP

Molecular Weight

Approximately 100-115 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

GRIK2 Protein, Human (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GRIK2 Protein, Human (HEK293, hFc)
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HY-P75156
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