GSTA1 Protein, Human (HEK293, His, solution)
Based on 1 Customer Validation
The GSTA1 protein catalyzes the attack of glutathione on various compounds, forming conjugates of PGA2 and PGJ2. It also converts AD into D4-androstene-3,17-dione, contributing to hormone biosynthesis. Additionally, it participates in the metabolism of oxidized linoleic acid through its peroxidase activity against 13-HPODE. GSTA1 Protein, Human (HEK293, His, solution) is the recombinant human-derived GSTA1 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The GSTA1 protein catalyzes the attack of glutathione on various compounds, forming conjugates of PGA2 and PGJ2. It also converts AD into D4-androstene-3,17-dione, contributing to hormone biosynthesis. Additionally, it participates in the metabolism of oxidized linoleic acid through its peroxidase activity against 13-HPODE. GSTA1 Protein, Human (HEK293, His, solution) is the recombinant human-derived GSTA1 protein, expressed by HEK293 , with C-His labeled tag.
Background
The GSTA1 Protein serves as a glutathione S-transferase, catalyzing the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a broad spectrum of both exogenous and endogenous compounds (Probable). This enzymatic activity includes the formation of glutathione conjugates for prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). Additionally, GSTA1 plays a role in hormone biosynthesis by catalyzing the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione. Notably, its glutathione-dependent peroxidase activity extends to the metabolism of oxidized linoleic acid, specifically targeting the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE. The diverse enzymatic functions of GSTA1 underscore its involvement in crucial cellular processes, from detoxification reactions to hormone biosynthesis and the metabolism of oxidized lipids.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P08263 (M1-F222)
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Molecular Construction
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N-term
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GSTA1 (M1-F222)
Accession # P08263 -
His
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C-term
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Protein Length
Full Length
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Synonyms
GSTA1; GTH1; Glutathione S-Transferase Alpha 1; Glutathione S-Transferase Ha Subunit 1; Glutathione S-Transferase A1; S-(Hydroxyalkyl)Glutathione Lyase A1; 13-Hydroperoxyoctadecadienoate Peroxidase; Glutathione S-Alkyltransferase A1; Androst-5-Ene-3,17-Di
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AA Sequence
MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF
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Predicted Molecular Mass
27.1 kDa
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Molecular Weight
Approximately 26 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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