HAAO Protein, Rat (His)

HAAO Protein, an enzyme, is involved in the metabolism of tryptophan and the production of quinolinic acid. Dysregulation of HAAO Protein has been associated with neurodegenerative diseases and psychiatric disorders. HAAO Protein, Rat (His) is the recombinant rat-derived HAAO protein, expressed by E. coli , with N-His labeled tag. The total length of HAAO Protein, Rat (His) is 286 a.a., with molecular weight of ~34.8 KDa.

The HAAO protein plays a crucial role in the biochemical pathway by catalyzing the oxidative ring opening of 3-hydroxyanthranilate, resulting in the formation of 2-amino-3-carboxymuconate semialdehyde. This semialdehyde compound then undergoes a spontaneous cyclization process, ultimately leading to the production of quinolinate.

Measured by its ability to inhibit the proliferation of PANC-1 Cells. The ED₅₀ for this effect is 0.106 μg/mL. Corresponding to a specific activity is 9.434×10³ Unit/mg.

Rat

E. coli

N-His

P46953 (M1-G286)

56823  [NCBI]

MERCVRVKSWVEENRASFQPPVCNKLMHREQLKIMFVGGPNTRKDYHIEEGEEVFYQLEGDMVLRVLEQGEHRDVVIRQGEIFLLPARVPHSPQRFANTMGLVIERRRMETELDGLRYYVGDTEDVLFEKWFHCKDLGTQLAPIIQEFFHSEQYRTGKPNPDQLLKEPPFPLSTRSVMEPMSLKAWLESHSRELQAGTSLSLFGDSYETQVIAHGQGSSKGPRQDVDVWLWQLEGSSKVTMGGQCVALAPDDSLLVPAGFSYMWERAQGSVALSVTQDPACKKPLG

Approximately 34.8 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.