HDAC8 Protein, Mouse (sf9, His)
The HDAC8 protein is a histone deacetylase that removes acetyl groups from core histone lysine residues, contributing to epigenetic repression and affecting transcriptional regulation, cell cycle progression, and developmental events. It forms large multiprotein complexes and deacetylates SMC3, regulating the release of cohesin complexes. HDAC8 Protein, Mouse (sf9, His) is the recombinant mouse-derived HDAC8 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The HDAC8 protein is a histone deacetylase that removes acetyl groups from core histone lysine residues, contributing to epigenetic repression and affecting transcriptional regulation, cell cycle progression, and developmental events. It forms large multiprotein complexes and deacetylates SMC3, regulating the release of cohesin complexes. HDAC8 Protein, Mouse (sf9, His) is the recombinant mouse-derived HDAC8 protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background
HDAC8 Protein is a histone deacetylase that facilitates the removal of acetyl groups from lysine residues on the N-terminal portion of core histones (H2A, H2B, H3, and H4). This deacetylation process contributes to epigenetic repression, thereby exerting significant influence on transcriptional regulation, cell cycle progression, and developmental events. The activity of HDAC8 is mediated through the formation of large multiprotein complexes. Furthermore, it participates in the deacetylation of cohesin complex protein SMC3, which regulates the release of cohesin complexes from chromatin. HDAC8 may also play a role in smooth muscle cell contractility. Additionally, apart from its deacetylase function, HDAC8 demonstrates protein-lysine deacylase activity by acting as a protein decrotonylase, catalyzing the removal of crotonyl groups ((2E)-butenoyl) from histones.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag C-His
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Accession
Q8VH37-1 (M1-V377)
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Molecular Construction
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N-term
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HDAC8 (M1-V377)
Accession # Q8VH37-1 -
His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
HDAC8; RPD3; Prev. HDACL1; Histone Deacetylase-Like 1; Prev. MRXS6; Wilson-Turner X-Linked Mental Retardation Syndrome; Prev. WTS; Histone Deacetylase; Protein Decrotonylase HDAC8; CDA07; Protein Deacetylase HDAC8; CDLS5; KDAC8; Histone Deacetylase 8; HD8
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AA Sequence
MEMPEEPANSGHSLPPVYIYSPEYVSICDSLVKVPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDEDHPDSIEYGLGYDCPATEGIFDYAAAIGGGTITAAQCLIDGKCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFDRILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDMSDVGLGKGRYYSVNVPIQDGIQDEKYYHICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYVLQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV
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Predicted Molecular Mass
43.1 kDa
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Molecular Weight
Approximately 46 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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