Copy Link Print Information

HER4 Protein, Rat (HEK293, His)

Customer Review

Based on 1 Customer Validation

HER4 Protein, crucial in central nervous system development, influences neural crest cell migration and axon guidance. Additionally, it participates in mammary gland differentiation, inducing milk proteins and contributing to lactation. HER4 Protein, Rat (HEK293, His) is the recombinant rat-derived HER4 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • HER4 Protein, Rat (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

HER4 Protein, crucial in central nervous system development, influences neural crest cell migration and axon guidance. Additionally, it participates in mammary gland differentiation, inducing milk proteins and contributing to lactation. HER4 Protein, Rat (HEK293, His) is the recombinant rat-derived HER4 protein, expressed by HEK293 , with C-His labeled tag.

Background

ERBB4 is a protein that plays important roles in the development of the central nervous system, neural crest cell migration, and axon guidance. It is also involved in mammary gland differentiation, induction of milk proteins, and lactation.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. The ED50 for this effect is 3.464 μg/mL in the presence of 10 ng/mL. Recombinant Human NRG1‑beta1, corresponding to a specific activity is 288.6836 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    Q62956-1 (Q26-P651)

  • Gene ID
    59323  [NCBI]
  • Molecular Construction
    • N-term
    • HER4 (Q26-P651)
      Accession # Q62956-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ERBB4; Proto-Oncogene-Like Protein C-ErbB-4; Erb-B2 Receptor Tyrosine Kinase 4; P180erbB4; HER4; V-Erb-A Avian Erythroblastic Leukemia Viral Oncogene Homolog-Like 4; ALS19; Avian Erythroblastic Leukemia Viral (V-Erb-B2) Oncogene Homolog 4; V-Erb-B2 Avian

  • AA Sequence

    QSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSIREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNMTLVSTIGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIDAIDPEKLNVFRTVREITGFLNIQTWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTVNQRIVIRDNRRAENCTAEGMVCNHLCSNDGCWGPGPDQCLSCRRFSRGKICIESCNLYDGEFREFENGSICVECDSQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDVLQGANSFIFKYADQDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTP

  • Molecular Weight

    Approximately 75-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • HER4 Protein, Rat (HEK293, His) Product Image
    1/16
  • HER4 Protein, Rat (HEK293, His) Product Image