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  4. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)

HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)

Cat. No.: HY-P75808
COA Handling Instructions

HEXB, or Hexosaminidase B protein, hydrolyzes N-acetyl-D-hexosamine in glycoconjugates. It selectively acts on neutral oligosaccharides, not degrading other substrates. HEXB does not degrade GM2 gangliosides; only isozyme A does. During fertilization, HEXB contributes to the zona block to polyspermy by inactivating the sperm galactosyltransferase-binding site. This mechanism prevents sperm binding to the zona pellucida. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is the recombinant mouse-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is 505 a.a., with molecular weight of ~59.4 kDa.

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Description

HEXB, or Hexosaminidase B protein, hydrolyzes N-acetyl-D-hexosamine in glycoconjugates. It selectively acts on neutral oligosaccharides, not degrading other substrates. HEXB does not degrade GM2 gangliosides; only isozyme A does. During fertilization, HEXB contributes to the zona block to polyspermy by inactivating the sperm galactosyltransferase-binding site. This mechanism prevents sperm binding to the zona pellucida. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is the recombinant mouse-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is 505 a.a., with molecular weight of ~59.4 kDa.

Background

HEXB, or Hexosaminidase B protein, plays a crucial role in hydrolyzing the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine found in glycoconjugates. This includes oligosaccharide moieties from proteins, neutral glycolipids, and certain mucopolysaccharides. Interestingly, isozyme B exhibits selective substrate specificity, efficiently hydrolyzing neutral oligosaccharides while not acting on other substrates. It is important to note that only isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. Additionally, during fertilization, HEXB contributes to the zona block to polyspermy, being present in the cortical granules of non-activated oocytes. Upon oocyte activation, HEXB is exocytosed during the cortical reaction, effectively inactivating the sperm galactosyltransferase-binding site. This molecular mechanism accounts for the block in sperm binding to the zona pellucida, as reported in studies.

Biological Activity

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide (4-MU-GlcNAc). Read at excitation and emission wavelengths of 365 nm and 445 nm (top read). The specific activity is 3033.81 pmol/min/µg, as measured under the described conditions.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P20060 (Q32-I536)

Gene ID

15212  [NCBI]

Synonyms
Beta-hexosaminidase subunit beta; HCC-7; HEXB
AA Sequence

QPALWPFPRSVQMFPRLLYISAEDFSIDHSPNSTAGPSCSLLQEAFRRYYNYVFGFYKRHHGPARFRAEPQLQKLLVSITLESECESFPSLSSDETYSLLVQEPVAVLKANSVWGALRGLETFSQLVYQDSFGTFTINESSIADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQSWGKGQKNLLTPCYNQKTKTQVFGPVDPTVNTTYAFFNTFFKEISSVFPDQFIHLGGDEVEFQCWASNPNIQGFMKRKGFGSDFRRLESFYIKKILEIISSLKKNSIVWQEVFDDKVELQPGTVVEVWKSEHYSYELKQVTGSGFPAILSAPWYLDLISYGQDWKNYYKVEPLNFEGSEKQKQLVIGGEACLWGEFVDATNLTPRLWPRASAVGERLWSPKTVTDLENAYKRLAVHRCRMVSRGIAAQPLYTGYCNYENKI

Molecular Weight

Approximately 59.4 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HEXB/Hexosaminidase B Protein, Mouse (HEK293, His)
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