HGF Protein, Rat (HEK293)

The HGF gene encodes hepatocyte growth factor (HGF), a protein involved in organ regeneration, angiogenesis, and myelination. It is located in the extracellular space and serves as a biomarker for cholangiocarcinoma. Implicated in various diseases, HGF plays a crucial role in physiological and pathological conditions. It shows biased expression in the liver (RPKM 137.5), lung (RPKM 132.1), and other tissues. HGF Protein, Rat (HEK293) is the recombinant rat-derived HGF protein, expressed by HEK293 , with tag free.

The HGF gene encodes hepatocyte growth factor (HGF), a protein that functions as a growth factor. HGF is involved in various biological processes, including animal organ regeneration, positive regulation of angiogenesis, and positive regulation of myelination. It is predominantly located in the extracellular space. HGF is utilized as a tool for studying brain infarction and serves as a biomarker for cholangiocarcinoma. Implicated in several diseases, including artery disease, autosomal recessive nonsyndromic deafness 39, various eye diseases, neuropathy, and multiple forms of scleroderma, the human ortholog of the HGF gene plays a crucial role in diverse physiological and pathological conditions. With biased expression observed in the liver (RPKM 137.5), lung (RPKM 132.1), and eight other tissues, HGF stands at the intersection of critical cellular processes and disease pathways.

1.Measured in a cell proliferation assay using Mv-1-1u mink lung epithelial cells and the ED₅₀ is typically 1-6 ng/mL.
2.Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED₅₀ for this effect is 10.41 ng/mL, corresponding to a specific activity is 9.606×10⁴ units/mg.

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  Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED₅₀ for this effect is 10.41 ng/mL, corresponding to a specific activity is 9.606×10⁴ units/mg.

Rat

HEK293

Tag Free

NP_058713.1 (Q33-L728)

24446  [NCBI]

Full Length of Mature Protein

QKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADECANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

Approximately 92-95 kDa & 60-62 kDa & 32-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.