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HGF Protein, Rat (HEK293)

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Based on 1 publication(s) in Google Scholar

The HGF gene encodes hepatocyte growth factor (HGF), a protein involved in organ regeneration, angiogenesis, and myelination. It is located in the extracellular space and serves as a biomarker for cholangiocarcinoma. Implicated in various diseases, HGF plays a crucial role in physiological and pathological conditions. It shows biased expression in the liver (RPKM 137.5), lung (RPKM 132.1), and other tissues. HGF Protein, Rat (HEK293) is the recombinant rat-derived HGF protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for HGF Protein, Rat (HEK293)
  • Experimental Validation Results for HGF Protein, Rat (HEK293)
  • HGF Protein, Rat (HEK293) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The HGF gene encodes hepatocyte growth factor (HGF), a protein involved in organ regeneration, angiogenesis, and myelination. It is located in the extracellular space and serves as a biomarker for cholangiocarcinoma. Implicated in various diseases, HGF plays a crucial role in physiological and pathological conditions. It shows biased expression in the liver (RPKM 137.5), lung (RPKM 132.1), and other tissues. HGF Protein, Rat (HEK293) is the recombinant rat-derived HGF protein, expressed by HEK293 , with tag free.

Background

The HGF gene encodes hepatocyte growth factor (HGF), a protein that functions as a growth factor. HGF is involved in various biological processes, including animal organ regeneration, positive regulation of angiogenesis, and positive regulation of myelination. It is predominantly located in the extracellular space. HGF is utilized as a tool for studying brain infarction and serves as a biomarker for cholangiocarcinoma. Implicated in several diseases, including artery disease, autosomal recessive nonsyndromic deafness 39, various eye diseases, neuropathy, and multiple forms of scleroderma, the human ortholog of the HGF gene plays a crucial role in diverse physiological and pathological conditions. With biased expression observed in the liver (RPKM 137.5), lung (RPKM 132.1), and eight other tissues, HGF stands at the intersection of critical cellular processes and disease pathways.

Verified Bioactivity

1.Measured in a cell proliferation assay using Mv-1-1u mink lung epithelial cells and the ED50 is typically 1-6 ng/mL.
2.Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED50 for this effect is 10.41 ng/mL, corresponding to a specific activity is 9.606×104 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag Tag Free
  • Accession

    NP_058713.1 (Q33-L728)

  • Gene ID
    24446  [NCBI]
  • Molecular Construction
    • N-term
    • HGF (Q33-L728)
      Accession # NP_058713.1
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    HGF; Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor); Prev. DFNB39; Lung Fibroblast-Derived Mitogen; HPTA; Hepatopoietin-A; SF; Scatter Factor; F-TCF; Deafness, Autosomal Recessive 39; HGFB; Hepatopoietin A; Hepatocyte Growth Factor; Fibroblast-D

  • AA Sequence

    QKKRRNTLHEFKKSAKTTLTKEDPLVKIKTKKVNSADECANRCIRNKGFPFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSAVNETDVPMETTECIKGQGEGYRGTTNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLTKNYCRNPDDDAHGPWCYTGNPLVPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

  • Predicted Molecular Mass

    79.3 kDa

  • Molecular Weight

    Approximately 92-95 kDa & 60-62 kDa & 32-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED50 for this effect is 10.41 ng/mL, corresponding to a specific activity is 9.606×104 units/mg.

  • HGF Protein, Rat (HEK293) Product Image
    1/16
  • Experimental Validation Results for HGF Protein, Rat (HEK293)
  • Experimental Validation Results for HGF Protein, Rat (HEK293)
  • HGF Protein, Rat (HEK293) Product Image