HJURP Protein, Human (His-SUMO)

The HJURP protein is a centromeric factor that plays a critical role in centromeric integration and maintenance of CENPA. As a chaperone, it ensures the integration of newly synthesized CENPA into nucleosomes at replicating centromeres. HJURP Protein, Human (His-SUMO) is the recombinant human-derived HJURP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HJURP Protein, Human (His-SUMO) is 748 a.a., with molecular weight of ~99.5 kDa.

HJURP, a centromeric protein, assumes a pivotal role in the intricate process of incorporating and maintaining the histone H3-like variant CENPA at centromeres. Functioning as a specific chaperone for CENPA, HJURP is indispensable for the integration of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Its regulatory prowess extends to preventing premature DNA binding by the CENPA-H4 tetramer and inhibiting CENPA-H4 tetramerization. HJURP's direct interaction with Holliday junctions further underscores its multifaceted involvement in centromeric dynamics. In molecular partnerships, HJURP forms a heterotrimer with CENPA and histone H4, where it interacts with the CENPA-H4 dimer, preventing tetramerization. Additionally, HJURP engages in phosphorylation-dependent interactions with 14-3-3 family members, emphasizing its intricate regulatory connections within the cellular machinery.

Human

E. coli

N-6*His;N-SUMO

Q8NCD3 (M1-V748)

55355  [NCBI]

MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQSPLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISRKSPGDPAKPASSPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPSSIISTKTFIMQNWNSRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRKTGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV

Approximately 99.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.