HJURP Protein, Human (His-SUMO)
Based on 1 Customer Validation
The HJURP protein is a centromeric factor that plays a critical role in centromeric integration and maintenance of CENPA. As a chaperone, it ensures the integration of newly synthesized CENPA into nucleosomes at replicating centromeres. HJURP Protein, Human (His-SUMO) is the recombinant human-derived HJURP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The HJURP protein is a centromeric factor that plays a critical role in centromeric integration and maintenance of CENPA. As a chaperone, it ensures the integration of newly synthesized CENPA into nucleosomes at replicating centromeres. HJURP Protein, Human (His-SUMO) is the recombinant human-derived HJURP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
Background
HJURP, a centromeric protein, assumes a pivotal role in the intricate process of incorporating and maintaining the histone H3-like variant CENPA at centromeres. Functioning as a specific chaperone for CENPA, HJURP is indispensable for the integration of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Its regulatory prowess extends to preventing premature DNA binding by the CENPA-H4 tetramer and inhibiting CENPA-H4 tetramerization. HJURP's direct interaction with Holliday junctions further underscores its multifaceted involvement in centromeric dynamics. In molecular partnerships, HJURP forms a heterotrimer with CENPA and histone H4, where it interacts with the CENPA-H4 dimer, preventing tetramerization. Additionally, HJURP engages in phosphorylation-dependent interactions with 14-3-3 family members, emphasizing its intricate regulatory connections within the cellular machinery.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;N-SUMO
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Accession
Q8NCD3-1 (M1-V748)
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Molecular Construction
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N-term
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6*His-SUMO
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HJURP (M1-V748)
Accession # Q8NCD3-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
HJURP; 14-3-3-Associated AKT Substrate; Holliday Junction Recognition Protein; Up-Regulated In Lung Cancer 9; URLC9; DKFZp762E1312; FAKTS; Fetal Liver Expressing Gene 1; HFLEG1; FLEG1; Fetal Liver-Expressing Gene 1 Protein
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AA Sequence
MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQSPLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISRKSPGDPAKPASSPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPSSIISTKTFIMQNWNSRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRKTGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV
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Predicted Molecular Mass
99.5 kDa
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Molecular Weight
Approximately 86 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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