HMGB3/HMG-3 Protein, Human (HEK293, His)

The multifunctional HMGB3/HMG-3 protein has multiple cellular functions and exhibits redox-sensitive behavior. It is associated with chromatin and exhibits a preference for non-canonical DNA structures, particularly single-stranded DNA. HMGB3/HMG-3 Protein, Human (HEK293, His) is the recombinant human-derived HMGB3/HMG-3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HMGB3/HMG-3 Protein, Human (HEK293, His) is 200 a.a., with molecular weight of ~26.0 kDa.

HMGB3/HMG-3, a versatile protein with diverse functions across cellular compartments, exhibits redox-sensitive behavior. It associates with chromatin, displaying a preference for non-canonical DNA structures, especially single-stranded DNA, and possesses the ability to bend DNA, enhancing flexibility through looping. This property provides a mechanism for influencing various gene promoters. Proposed to participate in the innate immune response to nucleic acids, HMGB3/HMG-3 may function as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Notably, it exerts negative regulatory effects on B-cell and myeloid cell differentiation, playing a crucial role in hematopoietic stem cells by potentially influencing the balance between self-renewal and differentiation. Additionally, HMGB3/HMG-3 is implicated in the negative regulation of canonical Wnt signaling, further emphasizing its multifaceted roles in cellular processes.

Human

HEK293

C-6*His

O15347 (M1-E200)

3149  [NCBI]

MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLNDSEKQPYITKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEDEEEEEEEEEEEEEEDE

Approximately 26.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 8% Trehalose, 2% Mannitol, 0.05% Tween 80, 2 mM EDTA, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.