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HMGB3/HMG-3 Protein, Human (HEK293, His)

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The multifunctional HMGB3/HMG-3 protein has multiple cellular functions and exhibits redox-sensitive behavior. It is associated with chromatin and exhibits a preference for non-canonical DNA structures, particularly single-stranded DNA. HMGB3/HMG-3 Protein, Human (HEK293, His) is the recombinant human-derived HMGB3/HMG-3 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The multifunctional HMGB3/HMG-3 protein has multiple cellular functions and exhibits redox-sensitive behavior. It is associated with chromatin and exhibits a preference for non-canonical DNA structures, particularly single-stranded DNA. HMGB3/HMG-3 Protein, Human (HEK293, His) is the recombinant human-derived HMGB3/HMG-3 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

HMGB3/HMG-3, a versatile protein with diverse functions across cellular compartments, exhibits redox-sensitive behavior. It associates with chromatin, displaying a preference for non-canonical DNA structures, especially single-stranded DNA, and possesses the ability to bend DNA, enhancing flexibility through looping. This property provides a mechanism for influencing various gene promoters. Proposed to participate in the innate immune response to nucleic acids, HMGB3/HMG-3 may function as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Notably, it exerts negative regulatory effects on B-cell and myeloid cell differentiation, playing a crucial role in hematopoietic stem cells by potentially influencing the balance between self-renewal and differentiation. Additionally, HMGB3/HMG-3 is implicated in the negative regulation of canonical Wnt signaling, further emphasizing its multifaceted roles in cellular processes.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    O15347 (M1-E200)

  • Gene ID
    3149  [NCBI]
  • Molecular Construction
    • N-term
    • HMGB3 (M1-E200)
      Accession # O15347
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    HMGB3; High Mobility Group Protein 4; Prev. HMG4; HMG-2a; HMG2A; HMG-4; High Mobility Group Protein B3; Non-Histone Chromosomal Protein; High Mobility Group Protein 2a; High-Mobility Group Box 3; High-Mobility Group (Nonhistone Chromosomal) Protein 4; Hig

  • AA Sequence

    MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSGKEKSKFDEMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLNDSEKQPYITKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEDEEEEEEEEEEEEEEDE

  • Predicted Molecular Mass

    24 kDa

  • Molecular Weight

    Approximately 26 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 8% Trehalose, 2% Mannitol, 0.05% Tween 80, 2 mM EDTA, pH 7.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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