HELPY Protein, Helicobacter pylori (T380A)
HpGT Protein, Helicobacter pylori is a glutathione-degrading enzyme that is a virulence factor in infection. Glutathione hydrolase proenzyme belongs to the gamma-glutamyltransferase family. pylorigamma-glutamyltranspeptidase is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity. HELPY Protein, Helicobacter pylori (T380A) is the recombinant HpGT protein, expressed by E. coli , with tag free.
- Species: Others
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HpGT Protein, Helicobacter pylori is a glutathione-degrading enzyme that is a virulence factor in infection. Glutathione hydrolase proenzyme belongs to the gamma-glutamyltransferase family. pylorigamma-glutamyltranspeptidase is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity[1][2]. HELPY Protein, Helicobacter pylori (T380A) is the recombinant HpGT protein, expressed by E. coli , with tag free.
Background
pylorigamma-glutamyltranspeptidase (HpGT) is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity. The resultant N terminus, Thr-380, has been shown to be the catalytic nucleophile in both the autoprocessing and enzymatic reactions. HpGT is a member of the N-terminal nucleophile hydrolase superfamily. HpGT has also been shown to up-regulate COX-2 and epidermal growth factor-related peptides in human gastric mucosal cells and to induce apoptosis in human gastric epithelial cells[1].
Helicobacter pylori γ-glutamyltranspeptidase (HpGT) catalyzes the first step in glutathione degradation, removing glutamate from the tripeptide and its conjugates via a γ-glutamylenzyme intermediate. The γ-glutamyl group can be transferred to water (hydrolysis) or an amino acid or short peptide (transpeptidation)[2].
Technical Parameters
-
Species Others
-
Source E. coli
-
Tag Tag Free
-
Accession
O25743 (S25-F567, T380A)
-
Gene ID/
-
Molecular Construction
-
N-term
-
HpGT (S25-F567, T380A)
Accession # O25743 -
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
Glutathione hydrolase proenzyme; EC:2.3.2.2; EC:3.4.19.13; Glutathione hydrolase large chain; Glutathione hydrolase small chain; HP_1118
-
AA Sequence
SAASYPPIKNTKVGLALSSHPLASEIGQKVLEEGGNAIDAAVAIGFALAVVHPAAGNIGGGGFAVIHLANGENVALDFREKAPLKATKNMFLDKQGNVVPKLSEDGYLAAGVPGTVAGMEAMLKKYGTKKLSQLIDPAIKLAENGYAISQRQAETLKEARERFLKYSSSKKYFFKKGHLDYQEGDLFVQKDLAKTLNQIKTLGAKGFYQGQVAELIEKDMKKNGGIITKEDLASYNVKWRKPVVGSYRGYKIISMSPPSSGGTHLIQILNVMENADLSALGYGASKNIHIAAEAMRQAYADRSVYMGDADFVSVPVDKLINKAYAKKIFDTIQPDTVTPSSQIKPGMGQLHEGSNATHYSVADRWGNAVSVTYTINASYGSAASIDGAGFLLNNEMDDFSIKPGNPNLYGLVGGDANAIEANKRPLSSMSPTIVLKNNKVFLVVGSPGGSRIITTVLQVISNVIDYNMNISEAVSAPRFHMQWLPDELRIEKFGMPADVKDNLTKMGYQIVTKPVMGDVNAIQVLPKTKGSVFYGSTDPRKEF
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
