Hydroxyacid oxidase 1 Protein, Mouse (His)
Hydroxy acid oxidase 1 (HAO1) is a multifunctional (S)-2-hydroxyacid oxidase with a strong preference for glycolate oxidation. This enzymatic process produces glyoxylic acid, an important precursor for the synthesis of glycine by alanine glyoxylate aminotransferase, which prevents glyoxylic acid accumulation and kidney stone formation. Hydroxyacid oxidase 1 Protein, Mouse (His) is the recombinant mouse-derived Hydroxyacid oxidase 1 protein, expressed by E. coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Hydroxy acid oxidase 1 (HAO1) is a multifunctional (S)-2-hydroxyacid oxidase with a strong preference for glycolate oxidation. This enzymatic process produces glyoxylic acid, an important precursor for the synthesis of glycine by alanine glyoxylate aminotransferase, which prevents glyoxylic acid accumulation and kidney stone formation. Hydroxyacid oxidase 1 Protein, Mouse (His) is the recombinant mouse-derived Hydroxyacid oxidase 1 protein, expressed by E. coli , with N-His labeled tag.
Background
Hydroxyacid oxidase 1 (HAO1) is a versatile (S)-2-hydroxy-acid oxidase with a broad substrate specificity, exhibiting a marked preference for glycolate oxidation. This enzymatic activity results in the production of glyoxylate, a crucial precursor essential for the peroxisomal synthesis of glycine by alanine-glyoxylate aminotransferase. This pathway plays a pivotal role in detoxifying glyoxylate, preventing its accumulation and the potential formation of kidney stones. HAO1 further displays the ability to catalyze the oxidation of glyoxylate and long-chain hydroxyacids, such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate. While active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), it is believed that O2 serves as the physiological electron acceptor, leading to the production of H2O2. HAO1's multifaceted enzymatic capabilities underscore its significance in various metabolic pathways.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
Q9WU19 (M1-I370)
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Molecular Construction
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N-term
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His
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(M1-I37)
Accession # Q9WU19 -
C-term
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Protein Length
Full Length
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Synonyms
HAO1; Glycolate Oxidase 1; Prev. GOX1; HAOX1; GOX; GO; Hydroxyacid Oxidase (Glycolate Oxidase) 1; Alternative Protein HAO1; (S)-2-Hydroxy-Acid Oxidase; Glycolate Oxidase; 2-Hydroxyacid Oxidase 1; Hydroxyacid Oxidase 1; Glyoxylate Oxidase
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AA Sequence
MLPRLVCISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKGILRGDDAKEAVKHGVDGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIIWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI
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Predicted Molecular Mass
45 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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