ICAM-5 Protein, Human (HEK293, His)

The ICAM-5 protein functions as a ligand for the leukocyte adhesion protein LFA-1 (integrin α-L/β-2) and mediates the cell adhesion process. As a member of the ICAM family, ICAM-5 interacts with LFA-1 and is critical for immune cell adhesion and communication. ICAM-5 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-5 protein, expressed by HEK293 , with C-His labeled tag.

ICAM-5 Protein serves as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2), indicating its role in mediating cellular adhesion processes. As a member of the ICAM protein family, ICAM-5 is implicated in interactions with LFA-1, which is crucial for the adhesion and communication between immune cells. This interaction likely plays a role in immune responses and cellular trafficking, contributing to the dynamic regulation of immune cell activities. The engagement of ICAM-5 with LFA-1 underscores its importance in the intricate network of adhesion molecules that modulate immune cell interactions and functions.

Measured by its ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5×10⁴ cells/well are added to ICAM-5 coated plates (25 μg/mL,100 μL/well) in the presence of 10 ng/mL PMA, approximately 42.83% will adhere after 2 hour at 37°C.

Human

HEK293

C-His

Q9UMF0 (E32-P834)

7087  [NCBI]

Partial

EPFWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTLSCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLGLFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGDAFVATATATASAEQEGARQLVCNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGAQALVTLEGVPAAVPGQPAQLQLNATENDDRRSFFCDATLDVDGETLIKNRSAELRVLYAPRLDDSDCPRSWTWPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLGPVTRALSGTYRCKAANDQGEAVKDVTLTVEYAPALDSVGCPERITWLEGTEASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPRGSAAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKCVGSGGATEGVLLPLAPPDPSPRAPRIPRVLAPGIYVCNATNRHGSVAKTVVVSAESPPEMDESTCPSHQTWLEGAEASALACAARGRPSPGVRCSREGIPWPEQQRVSREDAGTYHCVATNAHGTDSRTVTVGVEYRPVVAELAASPPGGVRPGGNFTLTCRAEAWPPAQISWRAPPGALNIGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGP

Approximately 112 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.