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IDI2 Protein, Human (His, solution)

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Based on 1 Customer Validation

IDI2 protein plays a crucial role in catalyzing the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP), leading to the formation of its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a key step in the biosynthetic pathway, contributing to the generation of essential building blocks for various cellular processes. IDI2 Protein, Human (His, solution) is the recombinant human-derived IDI2 protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IDI2 Protein, Human (His, solution)
  • IDI2 Protein, Human (His, solution) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

IDI2 protein plays a crucial role in catalyzing the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP), leading to the formation of its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a key step in the biosynthetic pathway, contributing to the generation of essential building blocks for various cellular processes. IDI2 Protein, Human (His, solution) is the recombinant human-derived IDI2 protein, expressed by E. coli , with N-6*His labeled tag.

Background

Isopentenyl-diphosphate Delta-isomerase 2 (IDI2) plays a pivotal role in isoprenoid biosynthesis by catalyzing the 1,3-allylic rearrangement of isopentenyl diphosphate (IPP), converting it to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a crucial step in the mevalonate pathway, which is responsible for the production of isoprenoids, essential compounds involved in various cellular processes, including protein prenylation and sterol biosynthesis. IDI2's ability to catalyze the isomerization of IPP to DMAPP is fundamental to the generation of diverse isoprenoid compounds that serve as precursors for a wide range of biomolecules in cellular metabolism.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q9BXS1 (M1-V227)

  • Gene ID
    91734  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • IDI2 (M1-V227)
      Accession # Q9BXS1
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    IDI2; Isopentenyl Pyrophosphate Isomerase 2; Isopentenyl-Diphosphate Delta Isomerase 2; IPP Isomerase 2; IPPI2; Diphosphate Dimethylallyl Diphosphate Isomerase 2; Isopentenyl-Diphosphate Delta-Isomerase 2

  • AA Sequence

    MSDINLDWVDRRQLQRLEEMLIVVDENDKVIGADTKRNCHLNENIEKGLLHRAFSVVLFNTKNRILIQQRSDTKVTFPGYFTDSCSSHPLYNPAELEEKDAIGVRRAAQRRLQAELGIPGEQISPEDIVFMTIYHHKAKSDRIWGEHEICYLLLVRKNVTLNPDPSETKSILYLSQEELWELLEREARGEVKVTPWLRTIAERFLYRWWPHLDDVTPFVELHKIHRV

  • Predicted Molecular Mass

    28.9 kDa

  • Molecular Weight

    Approximately 28-31 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 0.1 mM PMSF, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • IDI2 Protein, Human (His, solution) Product Image
    1/16
  • Experimental Validation Results for IDI2 Protein, Human (His, solution)
  • IDI2 Protein, Human (His, solution) Product Image