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IDO-2 Protein, Human (His)

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Based on 1 Customer Validation

The IDO-2 protein plays a key role in cellular processes, catalyzing the initial and rate-limiting steps in the catabolism of the essential amino acid tryptophan in the kynurenine pathway. This enzymatic activity has been confirmed in multiple studies, establishing IDO-2 as a key regulator of tryptophan metabolism with important implications for immune regulation. IDO-2 Protein, Human (His) is the recombinant human-derived IDO-2 protein, expressed by E. coli , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IDO-2 Protein, Human (His)
  • IDO-2 Protein, Human (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The IDO-2 protein plays a key role in cellular processes, catalyzing the initial and rate-limiting steps in the catabolism of the essential amino acid tryptophan in the kynurenine pathway. This enzymatic activity has been confirmed in multiple studies, establishing IDO-2 as a key regulator of tryptophan metabolism with important implications for immune regulation. IDO-2 Protein, Human (His) is the recombinant human-derived IDO-2 protein, expressed by E. coli , with C-6*His labeled tag.

Background

Indoleamine 2,3-dioxygenase 2 (IDO-2) is an enzyme that catalyzes the first and rate-limiting step in the catabolism of the essential amino acid tryptophan along the kynurenine pathway. This pathway is crucial for the regulation of tryptophan metabolism and has implications in immune regulation. IDO-2's activity contributes to immune modulation by limiting tryptophan availability, which, in turn, affects immune cell function. While IDO-2 is involved in immune regulation, it may not play a significant role in tryptophan-related tumoral resistance, as suggested by certain studies.

Verified Bioactivity

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >15 pmol/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    Q6ZQW0-1 (M1-G407)

  • Gene ID
    169355  [NCBI]
  • Molecular Construction
    • N-term
    • IDO-2 (M1-G407)
      Accession # Q6ZQW0-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    IDO2; Indoleamine 2,3-Dioxygenase-Like Protein 1; Prev. INDOL1; Alternative Protein IDO2; Indoleamine-Pyrrole 2,3-Dioxygenase-Like Protein 1; IDO-2; Indoleamine-Pyrrole 2,3 Dioxygenase-Like 1; Indoleamine 2,3-Dioxygenase 2; Indoleamine 2,3-Dioxygenase-Lik

  • AA Sequence

    MEPHRPNVKTAVPLSLESYHISEEYGFLLPDSLKELPDHYRPWMEIANKLPQLIDAHQLQAHVDKMPLLSCQFLKGHREQRLAHLVLSFLTMGYVWQEGEAQPAEVLPRNLALPFVEVSRNLGLPPILVHSDLVLTNWTKKDPDGFLEIGNLETIISFPGGESLHGFILVTALVEKEAVPGIKALVQATNAILQPNQEALLQALQRLRLSIQDITKTLGQMHDYVDPDIFYAGIRIFLSGWKDNPAMPAGLMYEGVSQEPLKYSGGSAAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCVQALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% Glycerol, 1 mM EDTA, 250 mM NaCl, pH 8.0.
2.Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • IDO-2 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for IDO-2 Protein, Human (His)
  • IDO-2 Protein, Human (His) Product Image