IGFBP-3 Protein, Human
Based on 1 Customer Validation
IGFBP-3 Protein, Human belongs to insulin-like growth factor-binding protein, prolongs the half-life of the insulin-like growth factors (IGFs), inhibits interaction of IGFs with their cell surface receptors, and functions in cancer reduction.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IGFBP-3 Protein, Human belongs to insulin-like growth factor-binding protein, prolongs the half-life of the insulin-like growth factors (IGFs), inhibits interaction of IGFs with their cell surface receptors, and functions in cancer reduction.
Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone[1]. Insulin-like Growth Factor-Binding Protein 3 (IGFBP3) is involved in prolonging the half-life of the IGFs (from 12 minutes to 12 hours), inhibits interaction of IGFs with their cell surface receptors, as well as, it is a circulating reservoir of IGFs. Moreover, IGFBP3 functions in cancer reduction, by both IGF-dependent and IGF-independent mechanisms. IGFBP3 has been shown to reduce the risk of breast cancer in high doses. A high concentration of IGFBP3 prevents IGF1:IGF1R interaction and blocks the IGF1R tyrosine kinase activity, resulting in the inhibition of the formation of intracellular signaling cascades[2].
The ED50 is <200 ng/mL as measured by serum free human MCF-7 cells, corresponding to a specific activity of >5.0 × 103 units/mg.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P17936-1 (G28-K291)
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Molecular Construction
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N-term
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IGFBP-3 (G28-K291)
Accession # P17936-1 -
C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
IGFBP3; Binding Protein 53; Insulin Like Growth Factor Binding Protein 3; Binding Protein 29; IBP3; IGFBP-3; Insulin-Like Growth Factor-Binding Protein 3; IBP-3; IGF-Binding Protein 3; CDNA FLJ43234 Fis, Clone HCHON2002964, Highly Similar To Insulin-Like
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AA Sequence
GASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK
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Predicted Molecular Mass
28.8 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705. [Content Brief]
[2]. Khodadadi E, et al. Cloning and expression of full-length human insulin-like growth factor binding protein 3 (IGFBP3) in the Escherichia coli. Adv Biomed Res. 2015 Mar 25;4:66. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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