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IGFBP-1 Protein, Mouse (SUMO, His)

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Based on 1 publication(s) in Google Scholar

The IGFBP-1 protein is an insulin-like growth factor binding protein that extends the half-life of IGF and modulates its growth-promoting effects in cell cultures, thereby inhibiting or stimulating these responses. Crucially, it alters the interaction between IGF and its cell surface receptors and promotes cell migration. IGFBP-1 Protein, Mouse (SUMO, His) is the recombinant mouse-derived IGFBP-1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IGFBP-1 Protein, Mouse (SUMO, His)
  • Experimental Validation Results for IGFBP-1 Protein, Mouse (SUMO, His)
  • IGFBP-1 Protein, Mouse (SUMO, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The IGFBP-1 protein is an insulin-like growth factor binding protein that extends the half-life of IGF and modulates its growth-promoting effects in cell cultures, thereby inhibiting or stimulating these responses. Crucially, it alters the interaction between IGF and its cell surface receptors and promotes cell migration. IGFBP-1 Protein, Mouse (SUMO, His) is the recombinant mouse-derived IGFBP-1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

Background

IGFBP-1, an insulin-like growth factor-binding protein, contributes to the regulation of IGFs by extending their half-life and modulating their growth-promoting effects on cell culture, either inhibiting or stimulating these effects. This protein plays a crucial role in altering the interaction between IGFs and their cell surface receptors. Additionally, IGFBP-1 demonstrates the ability to promote cell migration, indicating its involvement in cellular processes beyond growth regulation. Notably, it exhibits equal binding affinity for both IGF1 and IGF2, highlighting its versatile role in modulating the biological activities of insulin-like growth factors.

Verified Bioactivity

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is <1.5 µg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 1.317 µg/mL, corresponding to a specific activity is 759.3 U/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-6*His;N-SUMO
  • Accession

    P47876 (A26-N272)

  • Gene ID
    16006  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • IGFBP-1 (A26-N272)
      Accession # P47876
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IGFBP1; Alpha-Pregnancy-Associated Endometrial Globulin; Prev. IBP1; Amniotic Fluid Binding Protein; PP12; Binding Protein-28; Insulin-Like Growth Factor-Binding Protein 1; Binding Protein-26; IGF-Binding Protein 1; Binding Protein-25; Placental Protein 1

  • AA Sequence

    APQPWHCAPCTAERLGLCPPVPASCPEISRPAGCGCCPTCALPMGAACGVATARCAQGLSCRALPGEPRPLHALTRGQGACVPEPAAPATSTLFSSQHEEAKAAVVSADELSESPEMTEEQLLDSFHLMAPSREDQPILWNAISTYSSMRAREIADLKKWKEPCQRELYKVLERLAAAQQKAGDEIYKFYLPNCNKNGFYHSKQCETSLDGEAGLCWCVYPWSGKKIPGSLETRGDPNCHQYFNVHN

  • Molecular Weight

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl,0.5 M NaCI, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED50 for this effect is 1.317 µg/mL, corresponding to a specific activity is 759.3 U/mg.

  • IGFBP-1 Protein, Mouse (SUMO, His) Product Image
    1/16
  • Experimental Validation Results for IGFBP-1 Protein, Mouse (SUMO, His)
  • Experimental Validation Results for IGFBP-1 Protein, Mouse (SUMO, His)
  • IGFBP-1 Protein, Mouse (SUMO, His) Product Image