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IL-1RL2 Protein, Human (HEK293, His-Avi)

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IL-1RL2 Protein, serving as a receptor for interleukin-36 (IL36A, IL36B, and IL36G), forms a complex with the coreceptor IL1RAP, constituting the interleukin-36 receptor. This complex activates NF-kappa-B, MAPK, and other pathways, resembling the IL-1 system. IL-1RL2 is implicated in local inflammatory responses at epithelial barriers, particularly contributing to skin inflammatory reactions by inducing the IL-23/IL-17/IL-22 pathway. IL-1RL2 Protein, Human (HEK293, His-Avi) is the recombinant human-derived IL-1RL2 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

IL-1RL2 Protein, serving as a receptor for interleukin-36 (IL36A, IL36B, and IL36G), forms a complex with the coreceptor IL1RAP, constituting the interleukin-36 receptor. This complex activates NF-kappa-B, MAPK, and other pathways, resembling the IL-1 system. IL-1RL2 is implicated in local inflammatory responses at epithelial barriers, particularly contributing to skin inflammatory reactions by inducing the IL-23/IL-17/IL-22 pathway. IL-1RL2 Protein, Human (HEK293, His-Avi) is the recombinant human-derived IL-1RL2 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

IL-1RL2, a receptor for interleukin-36 (IL36A, IL36B, and IL36G), engages in complex signaling events upon binding to interleukin-36. Through association with the coreceptor IL1RAP, the interleukin-36 receptor complex is formed, orchestrating interleukin-36-dependent activation of NF-kappa-B, MAPK, and other pathways. This signaling system, akin to the IL-1 system, is implicated in local inflammatory responses within epithelial barriers, particularly contributing to skin inflammatory reactions by inducing the IL-23/IL-17/IL-22 pathway.

Verified Bioactivity

Immobilized Human IL-1 Rrp2, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-IL-1Rrp2 Antibody, hFc Tag with the EC50 of 12.4 ng/ml determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-8*His
  • Accession

    Q9HB29 (D20-R335)

  • Gene ID
    8808  [NCBI]
  • Molecular Construction
    • N-term
    • IL-1RL2 (D20-R335)
      Accession # Q9HB29
    • 8*His-Avi
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    IL1RL2; Interleukin-1 Receptor-Related Protein 2; Interleukin 1 Receptor Like 2; Interleukin-1 Receptor-Like 2; IL1R-Rp2; IL-36 Receptor; IL1RRP2; IL-1Rrp2; IL-36R; IL-1 Receptor-Related Protein 2

  • AA Sequence

    DGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFR

  • Molecular Weight

    Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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