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  4. IL-4 Receptor IL-13 Receptor IL-4R alpha/CD124
  5. IL-4R alpha/CD124
  6. IL-4R alpha/CD124 Protein, Rat (HEK293, His)

IL-4R alpha/CD124 Protein, Rat (HEK293, His)

Cat. No.: HY-P73217
COA Handling Instructions

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation. IL-4R alpha/CD124, Rat consists of 801 amino acids (M1-S801), exerts IL-4 binding function and results the phosphorylation of C-terminal tyrosine residues. IL-4R alpha/CD124, Rat has an immunoreceptor tyrosine inhibitor motif (ITIM) (698-703 a.a), which can bind to the SH2 domain of some phosphatases by phosphorylating ITIM. IL-4R alpha/CD124, Rat has a soluble form (1-229 a.a) and a transmembrane domain (233-256 a.a). The soluble IL-4R (sIL4R) inhibits IL4-induced spleen cell proliferation. IL-4R alpha/CD124 Protein, Rat is produced in HEK293 cells, including a Fibronectin typr III domain (125-223 a.a) and a C-Terminal His-tag.

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Description

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation[1]. IL-4R alpha/CD124, Rat consists of 801 amino acids (M1-S801), exerts IL-4 binding function and results the phosphorylation of C-terminal tyrosine residues. IL-4R alpha/CD124, Rat has an immunoreceptor tyrosine inhibitor motif (ITIM) (698-703 a.a), which can bind to the SH2 domain of some phosphatases by phosphorylating ITIM. IL-4R alpha/CD124, Rat has a soluble form (1-229 a.a) and a transmembrane domain (233-256 a.a). The soluble IL-4R (sIL4R) inhibits IL4-induced spleen cell proliferation. IL-4R alpha/CD124 Protein, Rat is produced in HEK293 cells, including a Fibronectin typr III domain (125-223 a.a) and a C-Terminal His-tag.

Background

Interleukin-4R alpha (IL-4Rα), also known as CD124 and B cell stimulatory factor (BSF) receptor, is one of the anti-inflammatory cytokines, and highly expressed in activated T-cells[1].
IL-4R alpha participates in forming two interleukin receptors in different cell types. For the type I receptor, depends on IL-4R alpha binding IL-4 to recruit IL-2R gamma chain in immune cells. IL-2R gamma is the common subunit for a variety of interleukin receptors, involved in the stimulation of neutrophil phagocytosis by IL-15. For the type II receptor, depends on IL-4R alpha binding IL-4 to recruit IL-13R alpha 1 chain. IL-13R alpha 1 is an alternat accessory protein to the common cytokine receptor gamma chain in non-immune cells[2][3].
The sequence of amino acids in IL-4R alpha proteins in rat shows low homology with human (52.82%) and mouse (79.53%).
IL-4 R alpha generates a soluble form by alternate splicing or proteolysis, maintaining ligand binding properties and inhibiting IL-4 bioactivity. IL-4 R alpha soluble isoform 1 can be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase[4].
IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis[5].

In Vitro

IL-4 R alpha shows a high R/S value in extracellular domain that indicates selective pressure for amino acid exchanges, and shows a low R/S value for the intracellular part[1].
IL-4 R alpha can be cleaved into a soluble interleukin-4 receptor, as sIL-4R, is expressed in kidney cells and antagonistically functional on IL-4-induced proliferation of spleen cells[2].
sIL-4R is found not only in rats spleen cells but also in their glomerular epithelial cells (GEC)[2].

In Vivo

IL-4 R alpha shows a high R/S value in extracellular domain that indicates selective pressure for amino acid exchanges, and shows a low R/S value for the intracellular part[6].
IL-4 R alpha can be cleaved into a soluble interleukin-4 receptor, as sIL-4R, is expressed in kidney cells and antagonistically functional on IL-4-induced proliferation of spleen cells[7].
sIL-4R is found not only in rats spleen cells but also in their glomerular epithelial cells (GEC)[7].

Biological Activity

Measured by its ability to bind mouse IL4-His in a functional ELISA.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q63257-1 (I26-R232)

Gene ID
Molecular Construction
N-term
IL-4Rα (I26-R232)
Accession # Q63257-1
hFc
C-term
Synonyms
Interleukin-4 receptor subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL4-BP; IL4R
Molecular Weight

Approximately 25.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

IL-4R alpha/CD124 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-4R alpha/CD124 Protein, Rat (HEK293, His)
Cat. No.:
HY-P73217
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