Integrin alpha 6 beta 1 Protein, Human (HEK293, His)

Measured by its binding ability in a functional ELISA. Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade with the EC₅₀ of 1.02 ng/mL.

Human

HEK293

C-His

P23229-2 (F24-G1012)&P05556-1 (Q21-D728)

3655&3688

Approximately 140-150 kDa (ITGA6) & 100-118 kDa(ITGB1),based on SDS-PAGE under non-reduced conditions,due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.