Integrin alpha A1 beta 1 Protein, Mouse (HEK293, His)
Integrin α-1/β-1 is a receptor for laminin and collagen that recognizes the proline hydroxylation sequence GFPGER within collagen. This integrin negatively regulates EGF-stimulated cell growth and forms a heterodimeric complex with beta-1 that interacts with the small GTPase RAB21. Integrin alpha A1 beta 1 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha A1 beta 1 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha A1 beta 1 Protein, Mouse (HEK293, His), has molecular weight of 140-200 kDa (ITGA1) & 100-115 kDa (ITGB1), respectively.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Integrin α-1/β-1 is a receptor for laminin and collagen that recognizes the proline hydroxylation sequence GFPGER within collagen. This integrin negatively regulates EGF-stimulated cell growth and forms a heterodimeric complex with beta-1 that interacts with the small GTPase RAB21. Integrin alpha A1 beta 1 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha A1 beta 1 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha A1 beta 1 Protein, Mouse (HEK293, His), has molecular weight of 140-200 kDa (ITGA1) & 100-115 kDa (ITGB1), respectively.
Background
The Integrin alpha-1/beta-1 protein functions as a receptor for both laminin and collagen, recognizing the proline-hydroxylated sequence G-F-P-G-E-R within collagen. Integral to anchorage-dependent processes, it negatively regulates EGF-stimulated cell growth. Forming a heterodimeric complex, Integrin alpha-1 associates with beta-1, and it interacts with the small GTPase RAB21. Moreover, through its cytoplasmic domain, Integrin alpha-1/beta-1 engages with PTPN2, activating the phosphatase activity of PTPN2 towards EGFR and thereby exerting a negative regulatory influence on EGF signaling pathways. These multifaceted interactions underscore Integrin alpha-1/beta-1's role in orchestrating cellular responses to the extracellular matrix and growth factor signaling.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-8*His
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Accession
Q3V3R4 (F29-P1141)&P09055-1 (Q21-D728)
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Gene ID109700&16412
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Molecular Construction
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N-term
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ITGA1 (F29-P1141)
Accession # Q3V3R4 -
8*His
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C-term
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N-term
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ITGB1 (Q21-D728)
Accession # P09055-1 -
C-term
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Synonyms
ITGA1; Very Late Antigen-1; Integrin Subunit Alpha 1; Integrin Alpha-1; CD49a; VLA-1; VLA1; Very Late Activation Protein 1; CD49 Antigen-Like Family Member A; Integrin, Alpha 1; Laminin And Collagen Receptor; CD49a Antigen; ITGB1; Very Late Activation Protein, Beta Polypeptide; Prev. MSK12; Integrin VLA-4 Beta Subunit; Prev. FNRB; VLA-4 Subunit Beta; Prev. MDF2; Integrin Beta 1; GPIIA; Integrin Beta; Integrin Beta-1; CD29 Antigen; CD29; VLA-BETA; Integrin, Beta 1 (Fibronectin Receptor, Beta Polypeptide, Antigen CD29 Includes MDF2, MSK12); VLAB; Glycoprotein IIa; Integrin Subunit Beta 1; Fibronectin Receptor Subunit Beta
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Predicted Molecular Mass
129 kDa (ITGA1) & 82.8 kDa (ITGB1)
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Molecular Weight
Approximately 150-200 kDa (ITGA1) & 90-120 kDa (ITGB1), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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