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Integrin alpha L beta 2 Protein, Mouse (HEK293, His)

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The integrin alpha L beta 2 protein is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, and F11R and is involved in immune phenomena, including leukocyte-endothelial cell interactions, cytotoxic T cell killing, and antibody-dependent killing. It contributes to natural killer cell cytotoxicity and leukocyte adhesion and migration. Integrin alpha L beta 2 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha L beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha L beta 2 Protein, Mouse (HEK293, His), has molecular weight of 160-180 kDa (ITGAL) & 90-105 kDa (ITGB2), respectively.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The integrin alpha L beta 2 protein is a receptor for ICAM1, ICAM2, ICAM3, ICAM4, and F11R and is involved in immune phenomena, including leukocyte-endothelial cell interactions, cytotoxic T cell killing, and antibody-dependent killing. It contributes to natural killer cell cytotoxicity and leukocyte adhesion and migration. Integrin alpha L beta 2 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha L beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha L beta 2 Protein, Mouse (HEK293, His), has molecular weight of 160-180 kDa (ITGAL) & 90-105 kDa (ITGB2), respectively.

Background

Integrin alpha L beta 2 (ITGAL/ITGB2) serves as a receptor for ICAM1, ICAM2, ICAM3, and ICAM4, and also interacts with the secreted form of ubiquitin-like protein ISG15 through its ITGAL subunit. Additionally, it functions as a receptor for F11R and plays a role in immune processes such as leukocyte-endothelial cell interaction, cytotoxic T-cell-mediated killing, and antibody-dependent killing by granulocytes and monocytes. ITGAL/ITGB2 contributes to natural killer cell cytotoxicity, as well as leukocyte adhesion and transmigration, involving T-cells and neutrophils. Its significance extends to the generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Furthermore, in association with ICAM3, ITGAL/ITGB2 contributes to apoptotic neutrophil phagocytosis by macrophages. The heterodimeric structure comprises an alpha subunit (ITGAL) and a beta subunit (ITGB2), with the alpha subunit interacting with THBD. This multifaceted functionality underscores the diverse roles of Integrin alpha L beta 2 in various immune and cellular processes.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    P24063-1 (Y24-H1087)&P11835 (Q24-N702)

  • Gene ID
    16408 & 16414
  • Synonyms

    ITGAL; Integrin Alpha-L; Prev. CD11A; HNA-5; LFA-1; Antigen CD11A (P180), Lymphocyte Function-Associated Antigen 1, Alpha Polypeptide; LFA1A; CDNA FLJ57691, Highly Similar To Integrin Alpha-L; Integrin, Alpha L (Antigen CD11A (P180), Lymphocyte Function-A

  • Predicted Molecular Mass

    123.3 kDa (ITGAL) & 79.6 kDa (ITGB2)

  • Molecular Weight

    Approximately 160-180 kDa (ITGAL) & 90-105 kDa (ITGB2), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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