IZUMO1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
IZUMO1 Protein, Human (HEK293, His) is the recombinant human-derived IZUMO1, expressed by HEK293 , with C-6*His labeled tag. ,
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IZUMO1 Protein, Human (HEK293, His) is the recombinant human-derived IZUMO1, expressed by HEK293 , with C-6*His labeled tag. ,
Background
IZUMO1 is an essential sperm cell-surface protein that functions in fertilization by acting as a ligand for the IZUMO1R/JUNO receptor on eggs. The IZUMO1:IZUMO1R/JUNO interaction represents a necessary adhesion event between sperm and egg, though it is insufficient for cell fusion and likely doesn't directly act as a membrane fusogen. During fertilization, IZUMO1 also serves as a ligand for the human-specific oolemma epitope FCRL3/MAIA (replacing IZUMO1R/JUNO as receptor), enabling species-specific gamete fusion. It plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. By similarity, IZUMO1 can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q8IYV9-1/AAH34769.1 (C22-R292)
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Molecular Construction
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N-term
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IZUMO1 (C22-R292)
Accession # Q8IYV9/AAH34769.1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IZUMO1; Izumo Sperm-Egg Fusion Protein 1; Izumo Sperm-Oocyte Fusion 1; Sperm-Specific Protein Izumo; OBF; Izumo Sperm-Egg Fusion 1; Oocyte Binding/Fusion Factor; MGC34799; IZUMO
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AA Sequence
CVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASR
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Molecular Weight
Approximately 43 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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