Kappa-Casein Protein, Human (HEK293, His)

Kappa-casein (CSN3) is a member of Caseins family, and is an extracellular mammalian milk protein involved in several important physiological processes. CSN3 prevents casein precipitation in milk and increase the efficiency of digestion, prevention of neonate hypersensitivity to ingested proteins, and inhibition of gastric pathogens. Kappa-Casein Protein, Human (HEK293, His) is the recombinant human-derived Kappa-Casein protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kappa-Casein Protein, Human (HEK293, His) is 162 a.a., with molecular weight of 28-35 kDa.

Kappa-casein (CSN3) is a member of Caseins family, and is an extracellular mammalian milk protein involved in several important physiological processes. CSN3 stabilizes micelle formation, preventing casein precipitation in milk. Chymosin splits CSN3 into an insoluble peptide (para kappa-casein) and water-soluble glycomacropeptide (GMP). GMP is responsible for an increased efficiency of digestion, prevention of neonate hypersensitivity to ingested proteins, and inhibition of gastric pathogens^[1][2].

Human

HEK293

C-6*His

AAH10935.1 (E21-A182)

1448  [NCBI]

EVQNQKQPACHENDERPFYQKTAPYVPMYYVPNSYPYYGTNLYQRRPAIAINNPCVPRTYYANPAVVRPHAQIPQRQYLPNSHPPTVVRRPNLHPSFIAIPPKKIQDKIIIPTINTIATVEPTPAPATEPTVDSVVTPEAFSESIITSTPETTTVAVTPPTA

28-35 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.