1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-10
  6. KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling)

KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling)

Cat. No.: HY-P78123
COA Handling Instructions

KGF-2/FGF-10 proteins coordinate embryonic development, regulate cell proliferation and differentiation, and are indispensable in branching morphogenesis. This multifunctional protein may aid in wound healing. KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling) is the recombinant human-derived KGF-2/FGF-10 protein, expressed by E. coli , with tag free. The total length of KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling) is 171 a.a., with molecular weight of ~19.3 kDa.

For research use only. We do not sell to patients.

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Description

KGF-2/FGF-10 proteins coordinate embryonic development, regulate cell proliferation and differentiation, and are indispensable in branching morphogenesis. This multifunctional protein may aid in wound healing. KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling) is the recombinant human-derived KGF-2/FGF-10 protein, expressed by E. coli , with tag free. The total length of KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling) is 171 a.a., with molecular weight of ~19.3 kDa.

Background

KGF-2/FGF-10 Protein assumes a crucial role in orchestrating embryonic development, exerting regulatory control over essential processes such as cell proliferation and differentiation. Its significance extends to the intricate domain of normal branching morphogenesis, where KGF-2/FGF-10 is indispensable. This versatile protein may also contribute to wound healing processes. Through crucial interactions, it engages with FGFR1 and FGFR2, forming molecular complexes that underlie its multifaceted functions. Furthermore, KGF-2/FGF-10 interacts with FGFBP1, emphasizing its intricate network of associations in orchestrating cellular responses and developmental events.

Biological Activity

Biotinylated Human FGF10, No tag immobilized on CM5 Chip can bind Human FGFR2 alpha lllb, His tag with an affinity constant of 147.70 nM as determined in SPR assay (Biacore T200).

Species

Human

Source

E. coli

Tag

Tag Free

Accession

O15520 (Q38-S208)

Gene ID
Molecular Construction
N-term
FGF-10 (Q38-S208)
Accession # O15520
C-term
Synonyms
FGF-10; KGF2
Molecular Weight

Approximately19.3 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl, pH 8.0. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
KGF-2/FGF-10 Protein, Human (Biotinylated, Primary Amine Labeling)
Cat. No.:
HY-P78123
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