KIRREL2/NEPH3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
KIRREL2/NEPH3 proteins may regulate basal insulin secretion and participate in self-association as homodimers. Functionally, it interacts with NPHS2/podocin, NPHS1 and FYN, contributing to key molecular interactions in cellular processes. KIRREL2/NEPH3 Protein, Human (HEK293, His) is the recombinant human-derived KIRREL2/NEPH3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
KIRREL2/NEPH3 proteins may regulate basal insulin secretion and participate in self-association as homodimers. Functionally, it interacts with NPHS2/podocin, NPHS1 and FYN, contributing to key molecular interactions in cellular processes. KIRREL2/NEPH3 Protein, Human (HEK293, His) is the recombinant human-derived KIRREL2/NEPH3 protein, expressed by HEK293 , with C-His labeled tag.
Background
KIRREL2/NEPH3 Protein is implicated in potentially regulating basal insulin secretion, suggesting its involvement in the intricate processes of insulin release. Structurally, it forms homodimers, indicative of its ability to engage in self-association. Functionally, KIRREL2/NEPH3 interacts with key proteins such as NPHS2/podocin, NPHS1, and FYN, highlighting its involvement in molecular interactions crucial for cellular processes. The interaction with NPHS2/podocin occurs through the C-terminus, while the interaction with NPHS1 involves the Ig-like domains, suggesting diverse binding mechanisms. Furthermore, the association with FYN adds a layer of complexity to its potential roles in cellular signaling pathways. The multifaceted interactions and potential regulatory roles of KIRREL2/NEPH3 underscore its significance in cellular functions beyond insulin secretion, warranting further investigation to unravel its precise contributions to various biological processes.
Verified Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of MS‑1 mouse pancreatic islet endothelial cells. When 5 x 10^4 cells per well are added to rhKirrel2 coated plates (30 μg/mL, 100 μL/well) approximately 68.481 % will adhere after 90 minutes at 37 °C.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q6UWL6-1 (G21-R503)
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Molecular Construction
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N-term
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KIRREL2 (G21-R503)
Accession # Q6UWL6-1 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
KIRREL2; Nephrin-Like Protein 3; Kirre Like Nephrin Family Adhesion Molecule 2; Nephrin-Like Gene 1; NEPH3; DKFZp564A1164; Kin Of Irregular Chiasm-Like Protein 2; MGC15718; Kin Of IRRE-Like Protein 2; Kin Of IRRE Like 2 (Drosophila); FILTRIN; Kin Of IRRE
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AA Sequence
GPSPHFLQQPEDLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAGLRSRPAQLHVLVPPEAPQVLGGPSVSLVAGVPANLTCRSRGDARPTPELLWFRDGVLLDGATFHQTLLKEGTPGSVESTLTLTPFSHDDGATFVCRARSQALPTGRDTAITLSLQYPPEVTLSASPHTVQEGEKVIFLCQATAQPPVTGYRWAKGGSPVLGARGPRLEVVADASFLTEPVSCEVSNAVGSANRSTALDVLFGPILQAKPEPVSVDVGEDASFSCAWRGNPLPRVTWTRRGGAQVLGSGATLRLPSVGPEDAGDYVCRAEAGLSGLRGGAAEARLTVNAPPVVTALHSAPAFLRGPARLQCLVFASPAPDAVVWSWDEGFLEAGSQGRFLVETFPAPESRGGLGPGLISVLHISGTQESDFSRSFNCSARNRLGEGGAQASLGRR
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Predicted Molecular Mass
52.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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