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Lacritin Protein, Human (His)

Cat. No.: HY-P70911
SDS COA Handling Instructions

Lacritin protein plays a pivotal role in modulating lacrimal acinar cell secretion. Lacritin Protein, Human (His) is the recombinant human-derived Lacritin protein, expressed by E. coli , with N-6*His labeled tag. The total length of Lacritin Protein, Human (His) is 120 a.a., with molecular weight of ~23.0 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $107 In-stock
50 μg $300 In-stock
100 μg   Get quote  

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  • Biological Activity

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Description

Lacritin protein plays a pivotal role in modulating lacrimal acinar cell secretion. Lacritin Protein, Human (His) is the recombinant human-derived Lacritin protein, expressed by E. coli , with N-6*His labeled tag. The total length of Lacritin Protein, Human (His) is 120 a.a., with molecular weight of ~23.0 kDa.

Background

Lacritin protein plays a pivotal role in the modulation of secretion by lacrimal acinar cells.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q9GZZ8 (A19-A138)

Gene ID
Molecular Construction
N-term
6*His
Lacritin (A19-A138)
Accession # Q9GZZ8
C-term
Synonyms
Extracellular Glycoprotein Lacritin; LACRT
AA Sequence

AEDASSDSTGADPAQEAGTSKPNEEISGPAEPASPPETTTTAQETSAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGGVPGGKQFIENGSEFAQKLLKKFSLLKPWA

Molecular Weight

Approximately 23.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Lacritin Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lacritin Protein, Human (His)
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HY-P70911
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