LAIR2 Protein, Human (HEK293)

Leukocyte-associated immunoglobulin-like receptor 2 is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 Protein, Human (HEK293) is the recombinant human-derived LAIR2 protein, expressed by HEK293 , with tag free.

Leukocyte-associated immunoglobulin-like receptor 2 is a member of the immunoglobulin superfamily. Leukocyte-associated immunoglobulin-like receptor 2 is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. Leukocyte-associated immunoglobulin-like receptor 2 maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily^[1][2][3].

Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rhLAIR-2 coated plates (50 µg/mL with 100 µL/well), approximately 43.3% will adhere after 10 minutes at 37°C.

Human

HEK293

Tag Free

Q6ISS4-1 (Q22-P152)

3904  [NCBI]

Full Length of Isoform-1 Mature Protein

QEGALPRPSISAEPGTVISPGSHVTFMCRGPVGVQTFRLEREDRAKYKDSYNVFRLGPSESEARFHIDSVSEGNAGLYRCLYYKPPGWSEHSDFLELLVKESSGGPDSPDTEPGSSAGTVPGTEASGFDAP

Approximately 22 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE. The apparent molecular mass of protein is approximately 22 kDa under reducing SDS-PAGE due to glycosylation.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Liu J, et al. Human-specific LAIR2 contributes to the high invasiveness of human extravillous trophoblast cells. Reprod Biol. 2019 Sep;19(3):287-292.  [Content Brief]

  [2]. Olde Nordkamp MJ,et al. Inhibition of the classical and lectin pathway of the complement system by recombinant LAIR-2. J Innate Immun. 2014;6(3):284-92.  [Content Brief]

  [3]. Founds SA, et al. LAIR2-expressing extravillous trophoblasts associate with maternal spiral arterioles undergoing physiologic conversion. Placenta. 2013 Mar;34(3):248-55.  [Content Brief]