LAIR2 Protein, Human (HEK293)
Based on 1 Customer Validation
Leukocyte-associated immunoglobulin-like receptor 2 is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 Protein, Human (HEK293) is the recombinant human-derived LAIR2 protein, expressed by HEK293 , with tag free.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Leukocyte-associated immunoglobulin-like receptor 2 is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 Protein, Human (HEK293) is the recombinant human-derived LAIR2 protein, expressed by HEK293 , with tag free.
Leukocyte-associated immunoglobulin-like receptor 2 is a member of the immunoglobulin superfamily. Leukocyte-associated immunoglobulin-like receptor 2 is a soluble receptor that may play roles in both inhibition of collagen-induced platelet aggregation and vessel formation during placental implantation. Leukocyte-associated immunoglobulin-like receptor 2 maps to a region of 19q13.4, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily[1][2][3].
Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with 100 µL/well), approximately 43.3% will adhere after 10 minutes at 37°C.
Technical Parameters
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Species Human
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Source HEK293
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Tag Tag Free
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Accession
Q6ISS4-1 (Q22-P152)
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Molecular Construction
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N-term
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LAIR2 (Q22-P152)
Accession # Q6ISS4-1 -
C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
LAIR2; Leukocyte-Associated Immunoglobulin-Like Receptor 2; Leukocyte Associated Immunoglobulin Like Receptor 2; Leukocyte-Associated Ig-Like Receptor 2; LAIR-2; Leukocyte-Associated Ig-Like Receptor-2; CD306; CD306 Antigen
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AA Sequence
QEGALPRPSISAEPGTVISPGSHVTFMCRGPVGVQTFRLEREDRAKYKDSYNVFRLGPSESEARFHIDSVSEGNAGLYRCLYYKPPGWSEHSDFLELLVKESSGGPDSPDTEPGSSAGTVPGTEASGFDAP
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Molecular Weight
Approximately 22 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu J, et al. Human-specific LAIR2 contributes to the high invasiveness of human extravillous trophoblast cells. Reprod Biol. 2019 Sep;19(3):287-292. [Content Brief]
[2]. Olde Nordkamp MJ,et al. Inhibition of the classical and lectin pathway of the complement system by recombinant LAIR-2. J Innate Immun. 2014;6(3):284-92. [Content Brief]
[3]. Founds SA, et al. LAIR2-expressing extravillous trophoblasts associate with maternal spiral arterioles undergoing physiologic conversion. Placenta. 2013 Mar;34(3):248-55. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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