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  4. LAIR2 Protein, Human (HEK293)

LAIR2 Protein, Human (HEK293)

Cat. No.: HY-P77050
COA Handling Instructions

For research use only. We do not sell to patients.

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10 μg $79 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 5 x 104 cells/well are added to rhLAIR-2 coated plates (50 µg/mL with 100 µL/well), approximately 43.3% will adhere after 10 minutes at 37°C.

Species

Human

Source

HEK293

Tag

Tag Free

Accession

Q6ISS4/NP_002279.2 (Q22-P152)

Gene ID
Molecular Construction
N-term
LAIR2 (Q22-P152)
Accession # Q6ISS4
C-term
Synonyms
Leukocyte-Associated Immunoglobulin-Like Receptor 2; LAIR-2; CD306
AA Sequence

QEGALPRPSISAEPGTVISPGSHVTFMCRGPVGVQTFRLEREDRAKYKDSYNVFRLGPSESEARFHIDSVSEGNAGLYRCLYYKPPGWSEHSDFLELLVKESSGGPDSPDTEPGSSAGTVPGTEASGFDAP

Molecular Weight

Approximately 22 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE. The apparent molecular mass of protein is approximately 22 kDa under reducing SDS-PAGE due to glycosylation.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LAIR2 Protein, Human (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LAIR2 Protein, Human (HEK293)
Cat. No.:
HY-P77050
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