LBP Protein, Human (HEK293, His)
Based on 1 Customer Validation
LBP proteins play a central role in the innate immune response by directly binding to the lipid A moiety of bacterial lipopolysaccharide (LPS). This interaction enhances CD14 affinity, promotes its binding to LPS, and promotes cytokine release in response to bacterial lipopolysaccharide. LBP Protein, Human (HEK293, His) is the recombinant human-derived LBP protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
LBP proteins play a central role in the innate immune response by directly binding to the lipid A moiety of bacterial lipopolysaccharide (LPS). This interaction enhances CD14 affinity, promotes its binding to LPS, and promotes cytokine release in response to bacterial lipopolysaccharide. LBP Protein, Human (HEK293, His) is the recombinant human-derived LBP protein, expressed by HEK293 , with C-6*His labeled tag.
LBP takes center stage in the innate immune response by directly engaging with the lipid A moiety of bacterial lipopolysaccharides (LPS), a pivotal glycolipid abundantly present in the outer membrane of Gram-negative bacteria. This interaction serves as a critical affinity enhancer for CD14, promoting its association with LPS and facilitating the subsequent release of cytokines in response to bacterial lipopolysaccharide. Notably, LBP, when bound to LPS, establishes interactions through its C-terminus with both soluble and membrane-bound CD14, underscoring its instrumental role in orchestrating key events in the innate immune system's defense against bacterial pathogens.
Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP 1 human acute monocytic leukemia cells. The ED50 for this effect is 0.4641-3.401 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P18428 (A26-V481)
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Molecular Construction
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N-term
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LBP (A26-V481)
Accession # P18428 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
LBP; BPIFD2; Lipopolysaccharide Binding Protein; BPI Fold Containing Family D, Member 2; Lipopolysaccharide-Binding Protein; LPS-Binding Protein
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AA Sequence
ANPGLVARITDKGLQYAAQEGLLALQSELLRITLPDFTGDLRIPHVGRGRYEFHSLNIHSCELLHSALRPVPGQGLSLSISDSSIRVQGRWKVRKSFFKLQGSFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFLFLGANVQYMRV
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Molecular Weight
Approximately 64 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 1 mM EDTA, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 1 mM EDTA, pH 8.0.
3.Lyophilized from a 0.22 μm filtered solution of PBS, 1 mM EDTA, pH 8.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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