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  5. LIFR Protein, Rat (HEK293, His)

LIFR Protein, Rat (HEK293, His)

Cat. No.: HY-P75907
COA Handling Instructions

The LIFR Protein, a vital member of the type I cytokine receptor family, mediates cellular responses to various cytokines, emphasizing its role in cell differentiation and immune regulation. Its study provides insights into the unique contributions of LIFR within the type I cytokine receptor family, offering potential applications in therapeutic interventions for conditions related to cell differentiation and immune response modulation. Further exploration holds promise for enhancing our knowledge of LIFR's role in both normal cellular function and pathological conditions. LIFR Protein, Rat (HEK293, His) is the recombinant rat-derived LIFR protein, expressed by HEK293, with C-His labeled tag. The total length of LIFR Protein, Rat (HEK293, His) is 829 a.a., with molecular weight of ~116 kDa.

For research use only. We do not sell to patients.

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Description

The LIFR Protein, a vital member of the type I cytokine receptor family, mediates cellular responses to various cytokines, emphasizing its role in cell differentiation and immune regulation. Its study provides insights into the unique contributions of LIFR within the type I cytokine receptor family, offering potential applications in therapeutic interventions for conditions related to cell differentiation and immune response modulation. Further exploration holds promise for enhancing our knowledge of LIFR's role in both normal cellular function and pathological conditions. LIFR Protein, Rat (HEK293, His) is the recombinant rat-derived LIFR protein, expressed by HEK293, with C-His labeled tag. The total length of LIFR Protein, Rat (HEK293, His) is 829 a.a., with molecular weight of ~116 kDa.

Background

The LIFR Protein is a significant member of the type I cytokine receptor family, specifically categorized within the Type 2 subfamily, highlighting its essential role in mediating cellular responses to various cytokines. As part of this receptor family, LIFR likely shares conserved structural and functional features with related receptors, emphasizing its involvement in transducing signals from specific type I cytokines. The classification within the type I cytokine receptor family underscores its specific designation within the broader context of cell signaling, providing insights into its unique contributions to processes such as cell differentiation and immune regulation. The study of LIFR contributes to our understanding of its role in physiological processes, offering potential applications in therapeutic interventions for conditions related to cell differentiation and immune response modulation. Further exploration of LIFR's role holds promise for enhancing our knowledge of its contributions to both normal cellular function and pathological conditions.

Biological Activity

1.Measured by its binding ability in a functional ELISA. Immobilized Mouse LIF hFc at 2 μg/mL (100 μl/well) can bind Rat LIFR His , the EC50 of Rat LIFR His is 300-700 ng/mL.
2.Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 5-40 ng/mL in the presence of 2 ng/mL recombinant human LIF.

Species

Rat

Source

HEK293

Tag

C-His

Accession

G3V7K2 (M1-S829)

Gene ID

81680  [NCBI]

Molecular Construction
N-term
LIFR (M1-S829)
Accession # G3V7K2
His
C-term
Synonyms
Leukemia inhibitory factor receptor; LIF-R; CD118
Molecular Weight

Approximately 116 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LIFR Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LIFR Protein, Rat (HEK293, His)
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