LOXL2 Protein, Human (HEK293, His, solution)
Based on 1 Customer Validation
The LOXL2 protein functions as a mediator in the post-translational oxidative deamination of lysine residues on target proteins, leading to the formation of deaminated lysine (allysine). Acting as a transcription corepressor, LOXL2 specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Notably, LOXL2 does not exhibit activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Additionally, LOXL2 mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, inducing the release of TAF10 from promoters and leading to the inhibition of TFIID-dependent transcription. This repression results in the downregulation of genes essential for embryonic stem cell pluripotency, including POU5F1/OCT4, NANOG, KLF4, and SOX2. LOXL2 is involved in epithelial to mesenchymal transition (EMT), participating in the repression of E-cadherin (CDH1) through the deamination of histone H3. It interacts with the endoplasmic reticulum protein HSPA5, activating the IRE1-XBP1 pathway of the unfolded protein response, and is implicated in E-cadherin repression following hypoxia, potentially contributing to tumor progression. Furthermore, when secreted into the extracellular matrix, LOXL2 promotes the cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. It also acts as a regulator of sprouting angiogenesis and chondrocyte differentiation.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The LOXL2 protein functions as a mediator in the post-translational oxidative deamination of lysine residues on target proteins, leading to the formation of deaminated lysine (allysine). Acting as a transcription corepressor, LOXL2 specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Notably, LOXL2 does not exhibit activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Additionally, LOXL2 mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, inducing the release of TAF10 from promoters and leading to the inhibition of TFIID-dependent transcription. This repression results in the downregulation of genes essential for embryonic stem cell pluripotency, including POU5F1/OCT4, NANOG, KLF4, and SOX2. LOXL2 is involved in epithelial to mesenchymal transition (EMT), participating in the repression of E-cadherin (CDH1) through the deamination of histone H3. It interacts with the endoplasmic reticulum protein HSPA5, activating the IRE1-XBP1 pathway of the unfolded protein response, and is implicated in E-cadherin repression following hypoxia, potentially contributing to tumor progression. Furthermore, when secreted into the extracellular matrix, LOXL2 promotes the cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. It also acts as a regulator of sprouting angiogenesis and chondrocyte differentiation.
Background
LOXL2 protein mediates post-translational oxidative deamination of lysine residues, leading to the formation of allysine. It specifically deaminates H3K4me3, inhibits TFIID-dependent transcription, represses E-cadherin, activates the IRE1-XBP1 pathway, and promotes cross-linking of extracellular matrix proteins. LOXL2 is involved in EMT, tumor progression, angiogenesis regulation, and chondrocyte differentiation.
Verified Bioactivity
Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >5 pmol/min/μg (QC tested).
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q9Y4K0 (Q26-Q774)
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Gene ID4017
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Molecular Construction
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N-term
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LOXL2 (Q26-Q774)
Accession # Q9Y4K0 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
LOXL2; Lysyl Oxidase-Related Protein 2; Lysyl Oxidase Like 2; Lysyl Oxidase-Like 2 Delta E13; Lysyl Oxidase-Like Protein 2; Lysyl Oxidase-Like 2 Protein; Lysyl Oxidase Homolog 2; Lysyl Oxidase Related 2; WS9-14; Lysyl Oxidase Homolog; LOR; Lysyl Oxidase-L
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AA Sequence
QYDSWPHYPEYFQQPAPEYHQPQAPANVAKIQLRLAGQKRKHSEGRVEVYYDGQWGTVCDDDFSIHAAHVVCRELGYVEAKSWTASSSYGKGEGPIWLDNLHCTGNEATLAACTSNGWGVTDCKHTEDVGVVCSDKRIPGFKFDNSLINQIENLNIQVEDIRIRAILSTYRKRTPVMEGYVEVKEGKTWKQICDKHWTAKNSRVVCGMFGFPGERTYNTKVYKMFASRRKQRYWPFSMDCTGTEAHISSCKLGPQVSLDPMKNVTCENGLPAVVSCVPGQVFSPDGPSRFRKAYKPEQPLVRLRGGAYIGEGRVEVLKNGEWGTVCDDKWDLVSASVVCRELGFGSAKEAVTGSRLGQGIGPIHLNEIQCTGNEKSIIDCKFNAESQGCNHEEDAGVRCNTPAMGLQKKLRLNGGRNPYEGRVEVLVERNGSLVWGMVCGQNWGIVEAMVVCRQLGLGFASNAFQETWYWHGDVNSNKVVMSGVKCSGTELSLAHCRHDGEDVACPQGGVQYGAGVACSETAPDLVLNAEMVQQTTYLEDRPMFMLQCAMEENCLSASAAQTDPTTGYRRLLRFSSQIHNNGQSDFRPKNGRHAWIWHDCHRHYHSMEVFTHYDLLNLNGTKVAEGHKASFCLEDTECEGDIQKNYECANFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESDYSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSPQ
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Predicted Molecular Mass
86.0 kDa
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Molecular Weight
Approximately 100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM MES, 50 mM NaCl, pH 6.5 with 10% trehalose as protectant.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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