MAN1A2 Protein, Human (HEK293, His)

The MAN1A2 protein is crucially involved in the maturation of Asn-linked oligosaccharides, which in turn trims α-1,2-linked mannose residues from Man(9)GlcNAc(2) to generate Man(5)GlcNAc(2). During glycosylation, MAN1A2 plays a key role in the progressive trimming of mannose residues that are essential for generating mature glycan structures. MAN1A2 Protein, Human (HEK293, His) is the recombinant human-derived MAN1A2 protein, expressed by HEK293 , with N-His labeled tag. The total length of MAN1A2 Protein, Human (HEK293, His) is 584 a.a., with molecular weight of ~69 KDa.

The MAN1A2 Protein plays a crucial role in the maturation of Asn-linked oligosaccharides by sequentially trimming alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to generate Man(5)GlcNAc(2). As part of the glycosylation process, MAN1A2 contributes to the stepwise modification of oligosaccharide structures, playing a key role in the progressive trimming of mannose residues. This enzymatic activity is pivotal for the generation of mature glycan structures, ensuring the proper functional maturation of glycoproteins. MAN1A2's involvement in oligosaccharide processing highlights its significance in the intricate network of cellular processes governing glycosylation and protein maturation.

Human

HEK293

N-His;N-10*His

O60476 (P58-R641)

10905  [NCBI]

PDSSKHKRFDLGLEDVLIPHVDAGKGAKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNKVVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHNELRPIARKGHSPNIFGSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVSVFEVNIRFIGGLLAAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR

Approximately 69 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.