MAPK4 Protein, Human (sf9, His, GST)
MAPK4, an atypical MAPK protein, phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5, forming a complex with intricate phosphorylation events. Interaction with MAPKAPK5 leads to ERK3/MAPK6 phosphorylation at Ser-189, activating MAPKAPK5, which reciprocally phosphorylates ERK3/MAPK6. The specific cellular role of this interplay, while unclear, suggests ERK3's potential involvement in promoting entry into the cell cycle. MAPK4 Protein, Human (sf9, His, GST) is the recombinant human-derived MAPK4 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
MAPK4, an atypical MAPK protein, phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5, forming a complex with intricate phosphorylation events. Interaction with MAPKAPK5 leads to ERK3/MAPK6 phosphorylation at Ser-189, activating MAPKAPK5, which reciprocally phosphorylates ERK3/MAPK6. The specific cellular role of this interplay, while unclear, suggests ERK3's potential involvement in promoting entry into the cell cycle. MAPK4 Protein, Human (sf9, His, GST) is the recombinant human-derived MAPK4 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Background
ERK3, an atypical MAPK protein, displays distinct kinase activity by phosphorylating microtubule-associated protein 2 (MAP2) and MAPKAPK5. The functional significance of the complex formed with MAPKAPK5 is not fully elucidated, but it involves a intricate series of phosphorylation events. Upon interaction with the atypical MAPKAPK5, ERK3/MAPK6 undergoes phosphorylation at Ser-189 and subsequently facilitates the phosphorylation and activation of MAPKAPK5. Intriguingly, MAPKAPK5 reciprocally phosphorylates ERK3/MAPK6. The specific role of this interplay in cellular processes remains unclear, although it is suggested that ERK3 may play a role in promoting entry into the cell cycle.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-8*His;N-GST
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Accession
P31152 (A2-W587)
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Gene ID5596
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Molecular Construction
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N-term
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8*His-GST
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MAPK4 (A2-W587)
Accession # P31152 -
C-term
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Protein Length
Full Length
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Synonyms
MAPK4; MAPK 4; Prev. PRKM4; Mitogen-Activated Protein Kinase; Erk3-Related; MAP Kinase Isoform P63; Erk4; MAP Kinase 4; Extracellular Signal-Regulated Kinase 4; P63MAPK; P63-MAPK; ERK4; Mitogen-Activated Protein Kinase 4; ERK-4
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AA Sequence
AEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAVDSRACRKVAVKKIALSDARSMKHALREIKIIRRLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYIHSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLFLEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNKLGDLNGACIPEHPGDLVQTEAFSKERW
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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