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MAX Protein, Human (His)

Cat. No.: HY-P70388
COA Handling Instructions

MAX protein is a transcriptional regulator that forms a complex with MYC to promote activation, or a complex with MAD to cause inhibition, binding to the core sequence 5'-CAC[GA]TG-3'. MAX acts as a repressor protein that recruits chromatin remodeling complexes with H3 "Lys-9" histone methyltransferase activity. MAX Protein, Human (His) is the recombinant human-derived MAX protein, expressed by E. coli , with C-6*His labeled tag. The total length of MAX Protein, Human (His) is 151 a.a., with molecular weight of ~21.0 kDa.

For research use only. We do not sell to patients.

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Description

MAX protein is a transcriptional regulator that forms a complex with MYC to promote activation, or a complex with MAD to cause inhibition, binding to the core sequence 5'-CAC[GA]TG-3'. MAX acts as a repressor protein that recruits chromatin remodeling complexes with H3 "Lys-9" histone methyltransferase activity. MAX Protein, Human (His) is the recombinant human-derived MAX protein, expressed by E. coli , with C-6*His labeled tag. The total length of MAX Protein, Human (His) is 151 a.a., with molecular weight of ~21.0 kDa.

Background

MAX, a transcription regulator, forms a sequence-specific DNA-binding protein complex either with MYC, promoting transcriptional activation, or with MAD, leading to transcriptional repression, recognizing the core sequence 5'-CAC[GA]TG-3'. MAX may act as a transcriptional repressor by recruiting a chromatin remodeling complex with H3 'Lys-9' histone methyltransferase activity and repressing MYC transcriptional activity from E-box elements. Efficient DNA binding requires dimerization with another bHLH protein. It binds DNA as a heterodimer with either MYC or MAD. MAX is part of the E2F6.com-1 complex during the G0 phase, along with various other components. It is also a component of some MLL1/MLL complexes, contributing to the regulation of gene expression. MAX interacts with SPAG9, and when in a heterodimer with MYC, it interacts with ABI1, potentially enhancing MYC:MAX transcriptional activity. MAX's versatility in forming complexes with different partners underscores its role as a key modulator of gene expression.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P61244-2 (M1-S151)

Gene ID
Molecular Construction
N-term
MAX (M1-S151)
Accession # P61244-2
6*His
C-term
Synonyms
rHuMAX, His; Protein Max; Class D Basic Helix-Loop-Helix Protein 4; bHLHd4; ; Myc-Associated Factor X; MAX; BHLHD4
AA Sequence

MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

Molecular Weight

Approximately 21.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, pH 8.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

MAX Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MAX Protein, Human (His)
Cat. No.:
HY-P70388
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