METTL11A Protein, Human
The METTL11A protein methylates the N-terminus of target proteins, specifically catalyzing monomethylation, dimethylation, or trimethylation of exposed α-amino groups in the [Ala/Gly/Ser]-Pro-Lys motif. METTL11A Protein, Human is the recombinant human-derived METTL11A protein, expressed by E. coli.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The METTL11A protein methylates the N-terminus of target proteins, specifically catalyzing monomethylation, dimethylation, or trimethylation of exposed α-amino groups in the [Ala/Gly/Ser]-Pro-Lys motif. METTL11A Protein, Human is the recombinant human-derived METTL11A protein, expressed by E. coli.
METTL11A protein functions as a distributive alpha-N-methyltransferase, exhibiting the capability to methylate the N-termini of target proteins that contain the specific N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys upon cleavage of the initiator methionine. This enzymatic activity results in mono-, di-, or tri-methylation of the exposed alpha-amino group of Ala, Gly, or Ser residues in the [Ala/Gly/Ser]-Pro-Lys motif, as well as mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some substrates may undergo priming by NTMT2-mediated monomethylation. METTL11A is responsible for the trimethylation of the N-terminal Gly in CENPA after the removal of Met-1 and plays a crucial role in the N-terminal methylation of various proteins, including KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A, and SET. During mitosis, METTL11A is required for normal bipolar spindle formation and chromosome segregation, exerting its effects through its action on RCC1. These diverse methylation activities highlight METTL11A's role in regulating crucial cellular processes and protein functions.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q9BV86-1 (T2-R223)
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Molecular Construction
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N-term
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METTL11A (T2-R223)
Accession # Q9BV86-1 -
C-term
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Protein Length
Partial
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Synonyms
NTMT1; Methyltransferase-Like Protein 11A; Prev. METTL11A; HOMT1A; Prev. C9orf32; N-Terminal RCC1 Methyltransferase; NRMT1; NTM1A; NRMT; Chromosome 9 Open Reading Frame 32; X-Pro-Lys N-Terminal Protein Methyltransferase 1A; Methyltransferase Like 11A; Alp
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AA Sequence
TSEVIEDEKQFYSKAKTYWKQIPPTVDGMLGGYGHISSIDINSSRKFLQRFLREGPNKTGTSCALDCGAGIGRITKRLLLPLFREVDMVDITEDFLVQAKTYLGEEGKRVRNYFCCGLQDFTPEPDSYDVIWIQWVIGHLTDQHLAEFLRRCKGSLRPNGIIVIKDNMAQEGVILDDVDSSVCRDLDVVRRIICSAGLSLLAEERQENLPDEIYHVYSFALR
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Predicted Molecular Mass
25.5 kDa
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Molecular Weight
Approximately 26 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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