MICB Protein, Human (HEK293, hFc)

In contrast to antigen presentation, MICB proteins serve as stress-induced autoantigens and are recognized by γδ T cells. As a ligand for the KLRK1/NKG2D receptor, binding of MICB results in cell lysis. MICB Protein, Human (HEK293, hFc) is the recombinant human-derived MICB protein, expressed by HEK293 , with C-hFc labeled tag.

MICB Protein does not play a role in antigen presentation; instead, it functions as a stress-induced self-antigen, recognized by gamma delta T cells. It serves as a ligand for the KLRK1/NKG2D receptor, and the binding of MICB to KLRK1 results in cell lysis. In contrast to classical MHC class I molecules, MICB does not form a heterodimer with beta-2-microglobulin but binds as a monomer to a KLRK1/NKG2D homodimer. The interaction between KLRK1 and MICB involves the formation of a complex with HCST/DAP10, where KLRK1 binds MICB, and HCST acts as an adapter molecule facilitating signal transduction. The receptor-ligand interaction induces the clustering of both proteins in ordered structures known as immune synapses and promotes their intercellular transfer, which is associated with a reduction in the cytotoxicity of KLRK1-expressing cells.

Human

HEK293

C-hFc

Q29980-1 (A23-G298)

4277  [NCBI]

Partial

AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSG

Approximately 70-80 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.