MICB Protein, Human (HEK293, hFc)
Based on 1 Customer Validation
In contrast to antigen presentation, MICB proteins serve as stress-induced autoantigens and are recognized by γδ T cells. As a ligand for the KLRK1/NKG2D receptor, binding of MICB results in cell lysis. MICB Protein, Human (HEK293, hFc) is the recombinant human-derived MICB protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
In contrast to antigen presentation, MICB proteins serve as stress-induced autoantigens and are recognized by γδ T cells. As a ligand for the KLRK1/NKG2D receptor, binding of MICB results in cell lysis. MICB Protein, Human (HEK293, hFc) is the recombinant human-derived MICB protein, expressed by HEK293 , with C-hFc labeled tag.
Background
MICB Protein does not play a role in antigen presentation; instead, it functions as a stress-induced self-antigen, recognized by gamma delta T cells. It serves as a ligand for the KLRK1/NKG2D receptor, and the binding of MICB to KLRK1 results in cell lysis. In contrast to classical MHC class I molecules, MICB does not form a heterodimer with beta-2-microglobulin but binds as a monomer to a KLRK1/NKG2D homodimer. The interaction between KLRK1 and MICB involves the formation of a complex with HCST/DAP10, where KLRK1 binds MICB, and HCST acts as an adapter molecule facilitating signal transduction. The receptor-ligand interaction induces the clustering of both proteins in ordered structures known as immune synapses and promotes their intercellular transfer, which is associated with a reduction in the cytotoxicity of KLRK1-expressing cells.
Verified Bioactivity
Immobilized Human MICB, hFc Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 30-80 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-hFc
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Accession
Q29980-1 (A23-G298)
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Molecular Construction
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N-term
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MICB (A23-G298)
Accession # Q29980-1 -
hFc
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
MICB; MHC Class I Chain-Related Protein; MHC Class I Polypeptide-Related Sequence B; Stress Inducible Class I Homolog; PERB11.2; MHC Class I Mic-B Antigen; MHC Class I Antigen-Related Protein B; MICB Protein; MHC Class I Chain-Related Protein B; MICB Anti
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AA Sequence
AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSG
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Predicted Molecular Mass
58.2 kDa
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Molecular Weight
Approximately 70-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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