MKNK2 Protein, Human (His)
Based on 1 Customer Validation
MKNK2 is a serine/threonine protein kinase that centrally affects cellular processes by phosphorylating SFPQ/PSF, HNRNPA1, and EIF4E. MKNK2 sits at the intersection of environmental stress and cytokine signaling and regulates translation by enhancing the affinity of EIF4E for the mRNA cap, thereby affecting the translation machinery. MKNK2 Protein, Human (His) is the recombinant human-derived MKNK2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
MKNK2 is a serine/threonine protein kinase that centrally affects cellular processes by phosphorylating SFPQ/PSF, HNRNPA1, and EIF4E. MKNK2 sits at the intersection of environmental stress and cytokine signaling and regulates translation by enhancing the affinity of EIF4E for the mRNA cap, thereby affecting the translation machinery. MKNK2 Protein, Human (His) is the recombinant human-derived MKNK2 protein, expressed by E. coli , with N-6*His labeled tag.
MKNK2, a serine/threonine-protein kinase, plays a pivotal role in cellular processes by phosphorylating SFPQ/PSF, HNRNPA1, and EIF4E. Positioned at the intersection of environmental stress and cytokine signaling, MKNK2 is implicated in the modulation of translation through the phosphorylation of EIF4E. This phosphorylation enhances EIF4E's affinity for the 7-methylguanosine-containing mRNA cap, thereby influencing the translational machinery. Moreover, MKNK2 is essential for mediating EIF4E shuttling from the cytoplasm to the nucleus. Notably, isoform 1 exhibits high basal kinase activity, while isoform 2 displays significantly lower kinase activity. MKNK2 serves as a key mediator in the suppressive effects of IFNgamma on hematopoiesis and acts as a negative regulator in signaling pathways governing arsenic trioxide As(2)O(3)-induced apoptosis and anti-leukemic responses. Additionally, MKNK2 is intricately involved in anti-apoptotic signaling triggered by serum withdrawal.
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q9HBH9 (T72-R385)
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Gene ID2872
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Molecular Construction
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N-term
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6*His
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MKNK2 (T72-R385)
Accession # Q9HBH9 -
C-term
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Protein Length
Partial
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Synonyms
MKNK2; G Protein-Coupled Receptor Kinase 7; Prev. GPRK7; CDNA FLJ10868 Fis, Clone NT2RP4001644, Weakly Similar To MYOSIN LIGHT CHAIN KINASE; MNK2; Putative Map Kinase Interacting Kinase; MAP Kinase-Interacting Serine/Threonine-Protein Kinase 2; MKNK2 Prot
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AA Sequence
TDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITSQEYAVKIIEKQPGHIRSRVFREVEMLYQCQGHRNVLELIEFFEEEDRFYLVFEKMRGGSILSHIHKRRHFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCEHPNQVSPVKICDFDLGSGIKLNGDCSPISTPELLTPCGSAEYMAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQR
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Predicted Molecular Mass
37.4 kDa
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Molecular Weight
Approximately 37 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
1.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol.
2.Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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