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Moesin Protein, Human (His)

Cat. No.: HY-P73806
COA Handling Instructions

Moesin proteins connect the actin cytoskeleton to the plasma membrane and regulate cell cortical structure and function. Moesin Protein, Human (His) is the recombinant human-derived Moesin protein, expressed by E. coli , with N-His labeled tag. The total length of Moesin Protein, Human (His) is 346 a.a., with molecular weight of ~45 kDa.

For research use only. We do not sell to patients.

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Description

Moesin proteins connect the actin cytoskeleton to the plasma membrane and regulate cell cortical structure and function. Moesin Protein, Human (His) is the recombinant human-derived Moesin protein, expressed by E. coli , with N-His labeled tag. The total length of Moesin Protein, Human (His) is 346 a.a., with molecular weight of ~45 kDa.

Background

Moesin, an Ezrin-radixin-moesin (ERM) family protein, serves as a crucial link between the actin cytoskeleton and the plasma membrane, thereby influencing the structure and function of specific domains in the cell cortex. Functioning as a tether for actin filaments, Moesin undergoes oscillation between a resting and an activated state, establishing transient interactions with the actin cytoskeleton. Upon phosphorylation of its C-terminal threonine, Moesin becomes activated, leading to interactions with F-actin and subsequent cytoskeletal rearrangements. These rearrangements play a pivotal role in regulating various cellular processes, including cell shape determination, membrane transport, and signal transduction. Moesin's significance is particularly pronounced in immunity, impacting both T and B-cell homeostasis and self-tolerance, and regulating lymphocyte egress from lymphoid organs. Additionally, Moesin modulates phagolysosomal biogenesis in macrophages and participates in immunologic synapse formation. The protein adopts a closed conformation (inactive form) through a head-to-tail association of its N-terminal and C-terminal halves, rendering it incapable of actin or membrane-binding in this state.

Species

Human

Source

E. coli

Tag

N-His

Accession

P26038 (M1-E346)

Gene ID
Molecular Construction
N-term
His
Moesin (M1-E346)
Accession # P26038
C-term
Synonyms
Moesin; Membrane-organizing extension spike protein; MSN
Molecular Weight

Approximately 45 kDa

Purity

Greater than 80% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.5M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Moesin Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Moesin Protein, Human (His)
Cat. No.:
HY-P73806
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