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MSP/MST1 Protein, Mouse (C677A, HEK293)

Cat. No.: HY-P701103
COA Handling Instructions

The MSP/MST1 protein, encoded by this gene, is predicted to have receptor tyrosine kinase binding activity. It is involved in various processes, including embryo implantation, mammary duct growth, and regulation of mammary gland cell proliferation. The protein is mainly found in the vacuole and expressed in tissues like the esophagus, liver, pharynx, and stomach. Notably, the gene shows liver-biased expression in both adult and embryonic stages. MSP/MST1 Protein, Mouse (C677A, HEK293) is the recombinant mouse-derived MSP/MST1 protein, expressed by HEK293 , with tag free. and C677A, , , , mutation.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The MSP/MST1 protein, encoded by this gene, is predicted to have receptor tyrosine kinase binding activity. It is involved in various processes, including embryo implantation, mammary duct growth, and regulation of mammary gland cell proliferation. The protein is mainly found in the vacuole and expressed in tissues like the esophagus, liver, pharynx, and stomach. Notably, the gene shows liver-biased expression in both adult and embryonic stages. MSP/MST1 Protein, Mouse (C677A, HEK293) is the recombinant mouse-derived MSP/MST1 protein, expressed by HEK293 , with tag free. and C677A, , , , mutation.

Background

This gene, encoding the MSP/MST1 protein, is predicted to possess receptor tyrosine kinase binding activity. It plays a role upstream in various processes, including embryo implantation, mammary duct terminal end bud growth, and positive regulation of mammary gland epithelial cell proliferation. The protein is primarily located in the vacuole and is expressed in tissues such as esophagus epithelium, liver, liver parenchyma, pharynx epithelium, and stomach proventricular region epithelium. Notably, the gene exhibits biased expression in the liver of both adult and embryonic stages in the reference dataset.

Biological Activity

Measured by its ability to inhibit the proliferation of Hela cell. The ED50 for this effect is 4.080 ng/ml, corresponding to a specific activity is 2.45×10^5 units/mg.

  • Measured by its ability to inhibit the proliferation of Hela cell. The ED50 for this effect  is 4.080 ng/mL, corresponding to a specific activity is 2.45×105 units/mg.
Species

Mouse

Source

HEK293

Tag

Tag Free

Accession

P26928/NP_032269 (Q19-E716, C677A)

Gene ID
Molecular Construction
N-term
MSP (Q19-E716, C677A)
Accession # P26928/NP_032269
C-term
Synonyms
Hepatocyte growth factor-like protein; MST1; Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; D3F15S2; DNF15S2; HGFL; Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like] ; Macrophage Stimulating Prot
AA Sequence

QRSPLNDFQLFRGTELRNLLHTAVPGPWQEDVADAEECARRCGPLLDCRAFHYNMSSHGCQLLPWTQHSLHTQLYHSSLCHLFQKKDYVRTCIMDNGVSYRGTVARTAGGLPCQAWSRRFPNDHKYTPTPKNGLEENFCRNPDGDPRGPWCYTTNRSVRFQSCGIKTCREAVCVLCNGEDYRGEVDVTESGRECQRWDLQHPHSHPFQPEKFLDKDLKDNYCRNPDGSERPWCYTTDPNVEREFCDLPSCGPNLPPTVKGSKSQRRNKGKALNCFRGKGEDYRGTTNTTSAGVPCQRWDAQSPHQHRFVPEKYACKDLRENFCRNPDGSEAPWCFTSRPGLRMAFCHQIPRCTEELVPEGCYHGSGEQYRGSVSKTRKGVQCQHWSSETPHKPQFTPTSAPQAGLEANFCRNPDGDSHGPWCYTLDPDILFDYCALQRCDDDQPPSILDPPDQVVFEKCGKRVDKSNKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWVLTARQCIWSCHEPLTGYEVWLGTINQNPQPGEANLQRVPVAKAVCGPAGSQLVLLKLERPVILNHHVALICLPPEQYVVPPGTKCEIAGWGESIGTSNNTVLHVASMNVISNQECNTKYRGHIQESEICTQGLVVPVGACEGDYGGPLACYTHDAWVLQGLIIPNRVCARPRWPAIFTRVSVFVDWINKVMQLE

Molecular Weight

92 & 60.5 & 32-36 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

MSP/MST1 Protein, Mouse (C677A, HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MSP/MST1 Protein, Mouse (C677A, HEK293)
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HY-P701103
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