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MSP/MST1 Protein, Mouse (C677A, HEK293)

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The MSP/MST1 protein, encoded by this gene, is predicted to have receptor tyrosine kinase binding activity. It is involved in various processes, including embryo implantation, mammary duct growth, and regulation of mammary gland cell proliferation. The protein is mainly found in the vacuole and expressed in tissues like the esophagus, liver, pharynx, and stomach. Notably, the gene shows liver-biased expression in both adult and embryonic stages. MSP/MST1 Protein, Mouse (C677A, HEK293) is the recombinant mouse-derived MSP/MST1 protein, expressed by HEK293 , with tag free, and C677A mutation.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MSP/MST1 Protein, Mouse (C677A, HEK293)
  • MSP/MST1 Protein, Mouse (C677A, HEK293) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The MSP/MST1 protein, encoded by this gene, is predicted to have receptor tyrosine kinase binding activity. It is involved in various processes, including embryo implantation, mammary duct growth, and regulation of mammary gland cell proliferation. The protein is mainly found in the vacuole and expressed in tissues like the esophagus, liver, pharynx, and stomach. Notably, the gene shows liver-biased expression in both adult and embryonic stages. MSP/MST1 Protein, Mouse (C677A, HEK293) is the recombinant mouse-derived MSP/MST1 protein, expressed by HEK293 , with tag free, and C677A mutation.

Background

This gene, encoding the MSP/MST1 protein, is predicted to possess receptor tyrosine kinase binding activity. It plays a role upstream in various processes, including embryo implantation, mammary duct terminal end bud growth, and positive regulation of mammary gland epithelial cell proliferation. The protein is primarily located in the vacuole and is expressed in tissues such as esophagus epithelium, liver, liver parenchyma, pharynx epithelium, and stomach proventricular region epithelium. Notably, the gene exhibits biased expression in the liver of both adult and embryonic stages in the reference dataset.

Verified Bioactivity

Measured by its ability to inhibit the proliferation of Hela cell. The ED50 for this effect is 4.080 ng/ml, corresponding to a specific activity is 2.45×10^5 units/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the proliferation of Hela cell. The ED50 for this effect is 4.080 ng/mL, corresponding to a specific activity is 2.45×105 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag Tag Free
  • Accession

    P26928/NP_032269 (Q19-E716, C677A)

  • Gene ID
    15235  [NCBI]
  • Molecular Construction
    • N-term
    • MSP (Q19-E716, C677A)
      Accession # P26928/NP_032269
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    MST1; NF15S2; Prev. D3F15S2; Macrophage Stimulatory Protein; Prev. DNF15S2; Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like); Prev. HGFL; Hepatocyte Growth Factor-Like Protein Homolog; MSP; Hepatocyte Growth Factor-Like; Hepatocyte Growth Factor-L

  • AA Sequence

    QRSPLNDFQLFRGTELRNLLHTAVPGPWQEDVADAEECARRCGPLLDCRAFHYNMSSHGCQLLPWTQHSLHTQLYHSSLCHLFQKKDYVRTCIMDNGVSYRGTVARTAGGLPCQAWSRRFPNDHKYTPTPKNGLEENFCRNPDGDPRGPWCYTTNRSVRFQSCGIKTCREAVCVLCNGEDYRGEVDVTESGRECQRWDLQHPHSHPFQPEKFLDKDLKDNYCRNPDGSERPWCYTTDPNVEREFCDLPSCGPNLPPTVKGSKSQRRNKGKALNCFRGKGEDYRGTTNTTSAGVPCQRWDAQSPHQHRFVPEKYACKDLRENFCRNPDGSEAPWCFTSRPGLRMAFCHQIPRCTEELVPEGCYHGSGEQYRGSVSKTRKGVQCQHWSSETPHKPQFTPTSAPQAGLEANFCRNPDGDSHGPWCYTLDPDILFDYCALQRCDDDQPPSILDPPDQVVFEKCGKRVDKSNKLRVVGGHPGNSPWTVSLRNRQGQHFCGGSLVKEQWVLTARQCIWSCHEPLTGYEVWLGTINQNPQPGEANLQRVPVAKAVCGPAGSQLVLLKLERPVILNHHVALICLPPEQYVVPPGTKCEIAGWGESIGTSNNTVLHVASMNVISNQECNTKYRGHIQESEICTQGLVVPVGACEGDYGGPLACYTHDAWVLQGLIIPNRVCARPRWPAIFTRVSVFVDWINKVMQLE

  • Predicted Molecular Mass

    53.7 kDa (α chain) & 25 kDa (β chain)

  • Molecular Weight

    Approximately 92 kDa & 61 kDa & 37 kDa & 32 kDa under reduced (R) conditions, 82 kDa under non reducing (N) conditions, based on SDS-PAGE.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its ability to inhibit the proliferation of Hela cell. The ED50 for this effect is 4.080 ng/mL, corresponding to a specific activity is 2.45×105 units/mg.

  • MSP/MST1 Protein, Mouse (C677A, HEK293) Product Image
    1/16
  • Experimental Validation Results for MSP/MST1 Protein, Mouse (C677A, HEK293)
  • MSP/MST1 Protein, Mouse (C677A, HEK293) Product Image