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Nectin-1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Nectin-1 Protein mediates cell-cell contacts through homophilic or heterophilic trans-dimer formation, engaging in heterophilic interactions with Nectin-3 and Nectin-4. It also promotes neurite outgrowth. In microbial infection, Nectin-1 serves as a receptor for herpes simplex virus 1 (HHV-1), herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV). Nectin-1 Protein, Human (HEK293, His) is the recombinant human-derived Nectin-1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Nectin-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Human (HEK293, His)
  • Nectin-1 Protein, Human (HEK293, His) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

Nectin-1 Protein mediates cell-cell contacts through homophilic or heterophilic trans-dimer formation, engaging in heterophilic interactions with Nectin-3 and Nectin-4. It also promotes neurite outgrowth. In microbial infection, Nectin-1 serves as a receptor for herpes simplex virus 1 (HHV-1), herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV). Nectin-1 Protein, Human (HEK293, His) is the recombinant human-derived Nectin-1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The Nectin-1 Protein facilitates cell-cell contacts through the formation of homophilic or heterophilic trans-dimers. It is known to engage in heterophilic interactions with Nectin-3 and Nectin-4. Additionally, this protein exhibits neurite outgrowth-promoting activity. In the context of microbial infection, Nectin-1 acts as a receptor for herpes simplex virus 1 (HHV-1), herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV).

Verified Bioactivity

1.Human Nectin-4 (HY-P70616) protein immobilized on CM5 Chip can bind Nectin-1 with an affinity constant of 41.47 nM as determined in SPR assay.
2.Loaded Anti-HumRecombinant Human Nectin-4 (C-Fc) on Pro-A Biosensor, can bind Recombinant Human Nectin-1 (C-6His) with an affinity constant of 67.1 nM as determined in BLI assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - SPR

    Bioactivity - SPR

    Human Nectin-4 (HY-P70616) protein immobilized on CM5 Chip can bind Nectin-1 with an affinity constant of 41.47 nM as determined in SPR assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q15223-1 (Q31-T334)

  • Gene ID
    5818  [NCBI]
  • Molecular Construction
    • N-term
    • Nectin-1 (Q31-T334)
      Accession # Q15223-1
    • 6*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    NECTIN1; OFC7; Prev. PVRL1; Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C); Prev. HVEC; Herpesvirus Ig-Like Receptor; Prev. ED4; Ectodermal Dysplasia 4 (Margarita Island Type); Nectin-1; Herpes Simplex Virus Type 1 Sensitivity; PRR1; Herpesv

  • AA Sequence

    QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRERVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAKKGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLACIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNIT

  • Predicted Molecular Mass

    35 kDa

  • Molecular Weight

    Approximately 40-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Human Nectin-4 (HY-P70616) protein immobilized on CM5 Chip can bind Nectin-1 with an affinity constant of 41.47 nM as determined in SPR assay.

  • Nectin-1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Nectin-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Human (HEK293, His)
  • Nectin-1 Protein, Human (HEK293, His) Product Image