Nectin-3/CD113 Protein, Human (HEK293, His-Avi)

Nectin-3/CD113, a versatile cell adhesion protein, mediates cell-cell interactions through heterophilic trans-binding with nectin-like proteins or nectins. It interacts with NECTIN2 at Sertoli-spermatid junctions, triggering CDC42 and RAC small G protein activation through common signaling molecules such as SRC and RAP1. Beyond adherens junctions and synapses, Nectin-3/CD113 induces endocytosis-mediated PVR down-regulation, reducing cell movement and proliferation. It contributes to ciliary body morphology, forms homodimers and heterodimers, and interacts with various proteins, including NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1, and IGSF4. The interaction with NECTIN1 at synaptic sites initiates puncta adherentia junction formation. Nectin-3/CD113 also binds to TIGIT with low affinity and interacts with AFDN, establishing a connection with the actin cytoskeleton. Nectin-3/CD113 Protein, Human (HEK293, His-Avi) is the recombinant human-derived Nectin-3/CD113 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Nectin-3/CD113 Protein, Human (HEK293, His-Avi) is 343 a.a., with molecular weight of 68-82 kDa.

Nectin-3/CD113, a versatile cell adhesion protein, plays a pivotal role in mediating cell-cell interactions through heterophilic trans-binding with nectin-like proteins or nectins, exemplified by its interaction with NECTIN2 at Sertoli-spermatid junctions. This trans-interaction with PVR triggers the activation of CDC42 and RAC small G proteins, mediated by common signaling molecules such as SRC and RAP1. Beyond its involvement in adherens junctions and synapses, Nectin-3/CD113 induces endocytosis-mediated down-regulation of PVR from the cell surface, leading to a reduction in cell movement and proliferation. Furthermore, it contributes to the morphology of the ciliary body and forms cis- and trans-homodimers, as well as trans-heterodimers with various proteins, including NECTIN1, NECTIN2, PVR, IGSF4B/Necl-1, and IGSF4. The interaction between NECTIN1 and NECTIN3 at pre- and postsynaptic sites initiates the formation of puncta adherentia junctions between axons and dendrites. Additionally, Nectin-3/CD113 binds with low affinity to TIGIT and interacts with AFDN, establishing a connection with the actin cytoskeleton.

Human

HEK293

C-Avi;C-His

Q9NQS3 (G58-D400)

25945  [NCBI]

68-82 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.