Neurofilament light polypeptide/NEFL, Mouse (His)
Based on 1 Customer Validation
Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons.It maintains neuronal caliber and assembles filamentous networks with PRPH and INA.Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E.coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons.It maintains neuronal caliber and assembles filamentous networks with PRPH and INA.Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E.coli , with N-His labeled tag.
Background
Neurofilament light polypeptide (NEFL), a component of neurofilaments, typically collaborates with the intermediate filament proteins NEFM and NEFH to maintain neuronal caliber, forming a structural foundation in neurons. In conjunction with neuronal intermediate filament proteins such as PRPH and INA, NEFL may participate in the assembly of neuronal filamentous networks. In vitro, NEFL demonstrates the ability to form homodimers and engage in heterodimerization with NEFH or NEFM, further forming hetero-oligomers. Additionally, NEFL forms heterodimers with INA. Its interactions extend to regulatory relationships, as evidenced by its association with ARHGEF28 and TRIM2, emphasizing the intricate molecular interplay involved in shaping neuronal structural integrity.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
P08551 (S2-D543)
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Molecular Construction
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N-term
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His
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NEFL (S2-D543)
Accession # P08551 -
C-term
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Protein Length
Full Length
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Synonyms
NEFL; Light Molecular Weight Neurofilament Protein; Neurofilament Light Chain; Neurofilament Light Polypeptide Isoform 1; NF68; Neurofilament Light Polypeptide Isoform 2; NFL; Neurofilament Protein, Light Chain; PPP1R110; Neurofilament, Light Polypeptide; CMT1F; 68 KDa Neurofilament Protein; CMT2E; Neurofilament Subunit NF-L; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament Light; Neurofilament, Light Polypeptide 68kDa; Neurofilament-Light; Neurofilament Light Polypeptide; NEFL Protein; Neurofilament Triplet L Protein; CMTDIG; NF-L
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AA Sequence
SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD
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Predicted Molecular Mass
65.5 kDa
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Molecular Weight
Approximately 68 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 6% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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