Neurofilament light polypeptide/NEFL, Mouse (His)

Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons. It maintains neuronal caliber and assembles filamentous networks with PRPH and INA. Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E. coli , with N-His labeled tag. The total length of Neurofilament light polypeptide/NEFL, Mouse (His) is 542 a.a., with molecular weight of ~68 kDa.

Neurofilament light polypeptide (NEFL), a component of neurofilaments, typically collaborates with the intermediate filament proteins NEFM and NEFH to maintain neuronal caliber, forming a structural foundation in neurons. In conjunction with neuronal intermediate filament proteins such as PRPH and INA, NEFL may participate in the assembly of neuronal filamentous networks. In vitro, NEFL demonstrates the ability to form homodimers and engage in heterodimerization with NEFH or NEFM, further forming hetero-oligomers. Additionally, NEFL forms heterodimers with INA. Its interactions extend to regulatory relationships, as evidenced by its association with ARHGEF28 and TRIM2, emphasizing the intricate molecular interplay involved in shaping neuronal structural integrity.

Mouse

E. coli

N-His

P08551 (S2-D543)

18039  [NCBI]

SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD

Approximately 68 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.