Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc)

Neurofilament light chain polypeptide (NEFL), like NEFM and NEFH, is an important component of neurofilaments and maintains neuronal caliber. NEFL interacts cooperatively with PRPH and INA to facilitate the assembly of neuronal filamentous networks. Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is the recombinant human-derived Neurofilament light polypeptide/NEFL, expressed by E. coli , with C-Myc, N-SUMO, N-10*His labeled tag. The total length of Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is 542 a.a., with molecular weight of ~81.4 kDa.

Neurofilament light polypeptide (NEFL) is a crucial component of neurofilaments, typically comprising a triad of intermediate filament proteins—NEFL, NEFM, and NEFH—that collectively contribute to the maintenance of neuronal caliber. NEFL may also engage in cooperative interactions with other neuronal intermediate filament proteins such as PRPH and INA, participating in the intricate assembly of neuronal filamentous networks. In addition to forming homodimers, NEFL forms heterodimers with NEFH or NEFM, and these complexes can further hetero-oligomerize. Furthermore, NEFL establishes interactions with specific proteins, including ARHGEF28 and TRIM2, highlighting its involvement in molecular networks that regulate various aspects of neuronal structure and function.

Human

E. coli

C-Myc;N-SUMO;N-10*His

P07196 (S2-D543)

4747  [NCBI]

SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

Approximately 81.4kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6%Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.