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Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc)

Cat. No.: HY-P72265
COA Handling Instructions

Neurofilament light chain polypeptide (NEFL), like NEFM and NEFH, is an important component of neurofilaments and maintains neuronal caliber. NEFL interacts cooperatively with PRPH and INA to facilitate the assembly of neuronal filamentous networks. Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is the recombinant human-derived Neurofilament light polypeptide/NEFL, expressed by E. coli , with C-Myc, N-SUMO, N-10*His labeled tag. The total length of Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is 542 a.a., with molecular weight of ~81.4 kDa.

For research use only. We do not sell to patients.

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Description

Neurofilament light chain polypeptide (NEFL), like NEFM and NEFH, is an important component of neurofilaments and maintains neuronal caliber. NEFL interacts cooperatively with PRPH and INA to facilitate the assembly of neuronal filamentous networks. Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is the recombinant human-derived Neurofilament light polypeptide/NEFL, expressed by E. coli , with C-Myc, N-SUMO, N-10*His labeled tag. The total length of Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) is 542 a.a., with molecular weight of ~81.4 kDa.

Background

Neurofilament light polypeptide (NEFL) is a crucial component of neurofilaments, typically comprising a triad of intermediate filament proteins—NEFL, NEFM, and NEFH—that collectively contribute to the maintenance of neuronal caliber. NEFL may also engage in cooperative interactions with other neuronal intermediate filament proteins such as PRPH and INA, participating in the intricate assembly of neuronal filamentous networks. In addition to forming homodimers, NEFL forms heterodimers with NEFH or NEFM, and these complexes can further hetero-oligomerize. Furthermore, NEFL establishes interactions with specific proteins, including ARHGEF28 and TRIM2, highlighting its involvement in molecular networks that regulate various aspects of neuronal structure and function.

Species

Human

Source

E. coli

Tag

C-Myc;N-SUMO;N-10*His

Accession

P07196 (S2-D543)

Gene ID
Molecular Construction
N-term
10*His-SUMO
NEFL (S2-D543)
Accession # P07196
C-term
Synonyms
CMT1F; CMT2E; NEFL; NF68
AA Sequence

SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

Molecular Weight

Approximately 81.4kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS, 6%Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Neurofilament light polypeptide/NEFL, Human (His-SUMO, myc)
Cat. No.:
HY-P72265
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