1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily Stem Cell CD Proteins
  4. TNF Receptor Superfamily Nerve Growth Factor Receptor (NGFR/CD271)
  5. Nerve Growth Factor Receptor (NGFR/CD271)
  6. NGFR Protein, Rabbit (HEK293, His)

NGFR Protein, Rabbit (HEK293, His)

Cat. No.: HY-P74699
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Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells. NGFR Protein, Human (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 250 amino acids (M1-N250) and is produced in HEK293 cells. NGFR Protein, Rabbit (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 242 amino acids (M1-D242) and is produced in HEK293 cells.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells[3]. NGFR Protein, Human (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 250 amino acids (M1-N250) and is produced in HEK293 cells. NGFR Protein, Rabbit (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 242 amino acids (M1-D242) and is produced in HEK293 cells.

Background

Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation[5].

Biological Activity

Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.163 µg/mL in the presence of 2 ng/mL of recombinant human beta-NGF, corresponding to a specific activity is 6.1350×10[3] units/mg.

  • Measured by its ability to inhibit beta-NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.163 µg/mL in the presence of 2 ng/mL of recombinant human beta-NGF, corresponding to a specific activity is 6.1350×103 units/mg.
Species

Rabbit

Source

HEK293

Tag

C-His

Accession

XP_008269543.1 (K22-D242)

Gene ID
Molecular Construction
N-term
NGFR (K22-D242)
Accession # XP_008269543.1
His
C-term
Synonyms
NGFR; Gp80-LNGFR; p75 ICD; CD271; TNFRSF16
AA Sequence

KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDTVCRCAYGYYQDETTGRCEAYRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECSRWADAECEAIPGRWTTRSPAPEGSDSMAPSTQVPEGPPEQELPASTAADVVTTVMGSSQPVATRGTAD

Molecular Weight

Approximately 43-50 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

NGFR Protein, Rabbit (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NGFR Protein, Rabbit (HEK293, His)
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HY-P74699
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