NOTCH1 Protein, Mouse (sf9, His)

NOTCH1 Protein acts as a receptor for membrane-bound ligands (JAG1, JAG2, and DLL1), determining cell fate.Upon activation, NICD forms a transcriptional activator complex, influencing differentiation, proliferation, and apoptosis.NOTCH1 negatively regulates angiogenesis, endothelial cell behavior, and impacts various developmental processes.It interacts with multiple partners, illustrating its involvement in signaling networks.NOTCH1 Protein, Mouse (sf9, His) is the recombinant mouse-derived NOTCH1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

NOTCH1 Protein functions as a receptor for membrane-bound ligands, including Jagged-1 (JAG1), Jagged-2 (JAG2), and Delta-1 (DLL1), playing a crucial role in cell-fate determination. Upon ligand activation, the released Notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, activating genes in the enhancer of split locus and influencing differentiation, proliferation, and apoptosis. NOTCH1 is involved in angiogenesis, negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. It contributes to thymic maturation of CD4(+) and CD8(+) cells, follicular differentiation, and possibly cell fate selection within the follicle. In cerebellar development, it serves as a receptor for neuronal DNER, impacting the differentiation of Bergmann glia. NOTCH1 also plays roles in repressing neuronal and myogenic differentiation, postimplantation development, mesoderm development, somite formation, and neurogenesis. Additionally, it modulates the balance between motile and immotile cilia for left/right symmetry determination. The protein interacts with various partners, including DNER, DTX1, DTX2, RBPJ/RBPSUH, MAML1, MAML2, MAML3, SNW1, AAK1, FBXW7, SGK1, HIF1AN, SNAI1, MDM2A, BCL6, THBS4, CCN3, DLL4, ZMIZ1, MEGF10, DLL1, JAG1, PRAG1, PSEN1, and ZFP64, highlighting its involvement in intricate signaling networks and regulatory processes.

Immobilized NOTCH1, Mouse (sf9, His) at 2 μg/mL (100 μL/well) can bind mouse DLL4-mFc, The EC₅₀ of NOTCH1, Mouse (sf9, His) is ≤0.2 μg/mL.

Mouse

Sf9 insect cells

C-His

Q01705 (A19-Q526)

18128  [NCBI]

Partial

ARGLRCSQPSGTCLNGGRCEVANGTEACVCSGAFVGQRCQDSNPCLSTPCKNAGTCHVVDHGGTVDYACSCPLGFSGPLCLTPLDNACLANPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFESSYICRCPPGFHGPTCRQDVNECSQNPGLCRHGGTCHNEIGSYRCACRATHTGPHCELPYVPCSPSPCQNGGTCRPTGDTTHECACLPGFAGQNCEENVDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFQGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECALGANPCEHAGKCLNTLGSFECQCLQGYTGPRCEIDVNECISNPCQNDATCLDQIGEFQCICMPGYEGVYCEINTDECASSPCLHNGHCMDKINEFQCQCPKGFNGHLCQ

Approximately 80 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.