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NTNG1 Protein, Human (HEK293, His)

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NTNG1, a master conductor in neural circuit development, intricately guides patterning across laminar, cellular, subcellular, and synaptic dimensions.Its influential role extends to fostering neurite outgrowth for axons and dendrites.In the symphony of neurodevelopment, NTNG1 interacts with NGL1, adding specificity to its involvement in shaping the intricate landscape of neuronal connectivity.NTNG1 Protein, Human (HEK293, His) is the recombinant human-derived NTNG1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

NTNG1, a master conductor in neural circuit development, intricately guides patterning across laminar, cellular, subcellular, and synaptic dimensions.Its influential role extends to fostering neurite outgrowth for axons and dendrites.In the symphony of neurodevelopment, NTNG1 interacts with NGL1, adding specificity to its involvement in shaping the intricate landscape of neuronal connectivity.NTNG1 Protein, Human (HEK293, His) is the recombinant human-derived NTNG1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

NTNG1 emerges as a master conductor in the orchestration of neural circuit development, intricately guiding patterning across laminar, cellular, subcellular, and synaptic dimensions. Its influential role extends to fostering the outgrowth of neurites, encompassing both axons and dendrites. In this symphony of neurodevelopment, NTNG1 engages in a nuanced interaction with NGL1, adding a layer of specificity to its involvement in shaping the intricate landscape of neuronal connectivity.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9Y2I2-1(H29-S409)

  • Gene ID
    22854  [NCBI]
  • Molecular Construction
    • N-term
    • NTNG1 (H29-S409)
      Accession # Q9Y2I2-1
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NTNG1; Netrin G1 Isoform G1n; Netrin G1; Netrin G1 Isoform G1m; Netrin-G1; Netrin G1 Isoform G1l; KIAA0976; Netrin G1 Isoform G1e; NetrinG1; Netrin G1 Isoform G1d; Lmnt1; Netrin G1 Isoform G1c; NetG1; Netrin G1 Isoform A; Laminet-1; Netrin G1f; Axon Guida

  • AA Sequence

    HYDLCKTQIYTEEGKVWDYMACQPESTDMTKYLKVKLDPPDITCGDPPETFCAMGNPYMCNNECDASTPELAHPPELMFDFEGRHPSTFWQSATWKEYPKPLQVNITLSWSKTIELTDNIVITFESGRPDQMILEKSLDYGRTWQPYQYYATDCLDAFHMDPKSVKDLSQHTVLEIICTEEYSTGYTTNSKIIHFEIKDRFAFFAGPRLRNMASLYGQLDTTKKLRDFFTVTDLRIRLLRPAVGEIFVDELHLARYFYAISDIKVRGRCKCNLHATVCVYDNSKLTCECEHNTTGPDCGKCKKNYQGRPWSPGSYLPIPKGTANTCIPSISSIGTNVCDNELLHCQNGGTCHNNVRCLCPAAYTGILCEKLRCEEAGSCGS

  • Predicted Molecular Mass

    43.5 kDa

  • Molecular Weight

    Approximately 51 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Histidine-HCl, 6% trehalose, 50 mM NaCl, 0.05% Tween 80, pH 5.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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