OCAM/NCAM2 Protein, Human (HEK293, His)

OCAM/NCAM2 proteins are potential key players in selective fasciculations and interregional projections in olfactory axons. Its specific mechanism and functional significance in guiding axonal development remain to be further elucidated. OCAM/NCAM2 Protein, Human (HEK293, His) is the recombinant human-derived OCAM/NCAM2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

OCAM/NCAM2 proteins are potential key players in selective fasciculations and interregional projections in olfactory axons. Its specific mechanism and functional significance in guiding axonal development remain to be further elucidated. OCAM/NCAM2 Protein, Human (HEK293, His) is the recombinant human-derived OCAM/NCAM2 protein, expressed by HEK293 , with C-His labeled tag.

Background

OCAM/NCAM2 Protein emerges as a potential key player, indicating its critical roles in selective fasciculation and zone-to-zone projection of primary olfactory axons. The specific mechanisms through which OCAM/NCAM2 contributes to these intricate processes remain to be fully elucidated, prompting further investigation into its functional significance in guiding the development and projection of olfactory axons. The proposed involvement of OCAM/NCAM2 in selective fasciculation underscores its potential impact on the formation of defined axonal bundles, while its role in zone-to-zone projection suggests a regulatory influence on the spatial organization of olfactory axons within distinct regions. The nuanced functions proposed for OCAM/NCAM2 highlight its significance in shaping the intricate neural circuitry associated with the primary olfactory system. Further research is essential to unravel the precise molecular pathways through which OCAM/NCAM2 orchestrates these crucial developmental events.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast cancer cells. The ED50 for this effect is 0.5143 μg/mL, corresponding to a specific activity is 1944.39 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • NCAM2 (L20-N697)
      Accession # O15394-1/NP_004531.2
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NCAM2; N-CAM-2; Neural Cell Adhesion Molecule 2; NCAM-2; NCAM21; Alternative Protein NCAM2; MGC51008

  • AA Sequence

    LLQVTISLSKVELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVVVQKEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREVVSPQEFKQGEDAEVVCRVSSSPAPAVSWLYHNEEVTTISDNRFAMLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAISMPQKSFNATAERGEEMTFSCRASGSPEPAISWFRNGKLIEENEKYILKGSNTELTVRNIINSDGGPYVCRATNKAGEDEKQAFLQVFVQPHIIQLKNETTYENGQVTLVCDAEGEPIPEITWKRAVDGFTFTEGDKSLDGRIEVKGQHGSSSLHIKDVKLSDSGRYDCEAASRIGGHQKSMYLDIEYAPKFISNQTIYYSWEGNPINISCDVKSNPPASIHWRRDKLVLPAKNTTNLKTYSTGRKMILEIAPTSDNDFGRYNCTATNHIGTRFQEYILALADVPSSPYGVKIIELSQTTAKVSFNKPDSHGGVPIHHYQVDVKEVASEIWKIVRSHGVQTMVVLNNLEPNTTYEIRVAAVNGKGQGDYSKIEIFQTLPVREPSPPSIHGQPSSGKSFKLSITKQDDGGAPILEYIVKYRSKDKEDQWLEKKVQGNKDHIILEHLQWTMGYEVQITAANRLGYSEPTVYEFSMPPKPNIIKDTLFN

  • Predicted Molecular Mass

    77.4 kDa

  • Molecular Weight

    Approximately 90 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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