p38 gamma/MAPK12 Protein, Human (sf9)

p38 gamma/MAPK12 Protein, a vital serine/threonine kinase in the MAP kinase pathway, responds to extracellular stimuli like pro-inflammatory cytokines or stress. Leading to the activation of transcription factors such as ELK1 and ATF2, p38 MAPKs phosphorylate around 200 to 300 substrates each. MAPK12 contributes to myoblast differentiation, down-regulates cyclin D1 in response to hypoxia, and inhibits cell proliferation while promoting differentiation. It phosphorylates DLG1, impacting mRNA processing during osmotic shock, regulates UV-induced checkpoint signaling, influences glucose uptake, ensures normal kinetochore localization of PLK1, and positively regulates myogenic precursor cell expansion in muscle growth and regeneration. p38 gamma/MAPK12 Protein, Human (sf9) is the recombinant human-derived p38 gamma/MAPK12 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of p38 gamma/MAPK12 Protein, Human (sf9) is 367 a.a., with molecular weight of ~43 kDa.

p38 gamma/MAPK12, a serine/threonine kinase and crucial component of the MAP kinase signal transduction pathway, is among the four p38 MAPKs playing a pivotal role in cellular responses triggered by extracellular stimuli such as pro-inflammatory cytokines or physical stress. This pathway leads to the direct activation of transcription factors like ELK1 and ATF2, with p38 MAPKs phosphorylating a diverse array of proteins, estimated to have approximately 200 to 300 substrates each. Among its downstream kinases is MAPKAPK2, activated through phosphorylation to further phosphorylate additional targets. MAPK12 contributes to myoblast differentiation and down-regulation of cyclin D1 in response to hypoxia in adrenal cells, suggesting its role in inhibiting cell proliferation while promoting differentiation. Notably, MAPK12 phosphorylates DLG1, and following osmotic shock, it increases its association with nuclear DLG1, impacting mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes. Additionally, MAPK12 regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage, influences glucose uptake in muscle cells, and is essential for the normal kinetochore localization of PLK1, preventing chromosomal instability and supporting mitotic cell viability. Its signaling positively regulates the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

Tag Free

P53778 (N-G&P, M1-L367)

6300  [NCBI]

Approximately 43 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.