P4HB Protein, Mouse (HEK293, His)

P4HB proteins are versatile and multifunctional, coordinating disulfide bond catalysis at the cell surface and inside the cell. It exhibits reductase activity, cleaves disulfide bonds, induces structural modifications, and functions as a chaperone to inhibit aggregation of misfolded proteins. P4HB Protein, Mouse (HEK293, His) is the recombinant mouse-derived P4HB protein, expressed by HEK293 , with C-His labeled tag. The total length of P4HB Protein, Mouse (HEK293, His) is 490 a.a., with molecular weight of 57-70 kDa.

P4HB Protein, a versatile and multifunctional entity, orchestrates the catalysis of disulfide bond formation, breakage, and rearrangement. Positioned at the cell surface, it exhibits reductase activity, cleaving disulfide bonds of proteins attached to the cell and potentially inducing structural modifications in exofacial proteins. Within the cellular interior, P4HB plays a role in forming and rearranging disulfide bonds in nascent proteins. At elevated concentrations, and upon phosphorylation by FAM20C, it transforms into a chaperone that inhibits the aggregation of misfolded proteins, whereas at lower concentrations, it facilitates aggregation, showcasing anti-chaperone activity. Furthermore, P4HB may collaborate with other chaperones in modifying the structure of the thyroglobulin precursor during hormone biogenesis. Additionally, it serves as a structural subunit for various enzymes, including prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Notably, P4HB also functions as a receptor for LGALS9, with this interaction impacting its localization on the cell surface, enhancing disulfide reductase activity, altering the plasma membrane redox state, and promoting cell migration.

Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A₆₅₀/cm/min/mg.

Mouse

HEK293

C-His

P09103 (D20-L509)

18453  [NCBI]

DALEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKRAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDEDLDLEEALEPDMEEDDDQKAVKDEL

57-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.