1. Recombinant Proteins
  2. Others
  3. P4HB Protein, Mouse (HEK293, His)

P4HB Protein, Mouse (HEK293, His)

Cat. No.: HY-P74658
COA Handling Instructions

P4HB proteins are versatile and multifunctional, coordinating disulfide bond catalysis at the cell surface and inside the cell.It exhibits reductase activity, cleaves disulfide bonds, induces structural modifications, and functions as a chaperone to inhibit aggregation of misfolded proteins.P4HB Protein, Mouse (HEK293, His) is the recombinant mouse-derived P4HB protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $56 In-stock
10 μg $95 In-stock
50 μg $265 In-stock
100 μg $450 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

P4HB proteins are versatile and multifunctional, coordinating disulfide bond catalysis at the cell surface and inside the cell.It exhibits reductase activity, cleaves disulfide bonds, induces structural modifications, and functions as a chaperone to inhibit aggregation of misfolded proteins.P4HB Protein, Mouse (HEK293, His) is the recombinant mouse-derived P4HB protein, expressed by HEK293 , with C-His labeled tag.

Background

P4HB Protein, a versatile and multifunctional entity, orchestrates the catalysis of disulfide bond formation, breakage, and rearrangement. Positioned at the cell surface, it exhibits reductase activity, cleaving disulfide bonds of proteins attached to the cell and potentially inducing structural modifications in exofacial proteins. Within the cellular interior, P4HB plays a role in forming and rearranging disulfide bonds in nascent proteins. At elevated concentrations, and upon phosphorylation by FAM20C, it transforms into a chaperone that inhibits the aggregation of misfolded proteins, whereas at lower concentrations, it facilitates aggregation, showcasing anti-chaperone activity. Furthermore, P4HB may collaborate with other chaperones in modifying the structure of the thyroglobulin precursor during hormone biogenesis. Additionally, it serves as a structural subunit for various enzymes, including prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Notably, P4HB also functions as a receptor for LGALS9, with this interaction impacting its localization on the cell surface, enhancing disulfide reductase activity, altering the plasma membrane redox state, and promoting cell migration.

Biological Activity

Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A650/cm/min/mg.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P09103 (D20-L509)

Gene ID
Molecular Construction
N-term
P4HB (D20-L509)
Accession # P09103
His
C-term
Synonyms
Protein disulfide-isomerase; PDI; ERp59; p55; P4HB; Pdia1
AA Sequence

DALEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKDGVVLFKKFDEGRNNFEGEITKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKRAAEGFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDEDLDLEEALEPDMEEDDDQKAVKDEL

Molecular Weight

57-70 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

P4HB Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
P4HB Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74658
Quantity:
MCE Japan Authorized Agent: