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PADI2 Protein, Human (HEK293, His)

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The PADI2 protein serves as a catalyst for the deimination process, specifically targeting arginine residues within proteins and facilitating their conversion. PADI2 Protein, Human (HEK293, His) is the recombinant human-derived PADI2 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PADI2 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The PADI2 protein serves as a catalyst for the deimination process, specifically targeting arginine residues within proteins and facilitating their conversion. PADI2 Protein, Human (HEK293, His) is the recombinant human-derived PADI2 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

PADI2 Protein, an enzyme, plays a crucial role in post-translational modifications of proteins. Dysregulation of PADI2 Protein has been associated with autoimmune disorders and inflammatory diseases. Targeting PADI2 Protein may offer potential therapeutic interventions in these conditions by modulating protein modifications, suppressing autoimmune responses, and managing inflammatory disorders.

Verified Bioactivity

Measured by its ability to catalyze the deimination of 10mM benzoyl-arginine ethyl ester that incubate at 37°C for 60minutes.The specific activity is 12.383 pmol/min/µg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q9Y2J8-1 (M1-P665)

  • Gene ID
    11240  [NCBI]
  • Molecular Construction
    • N-term
    • PADI2 (M1-P665)
      Accession # Q9Y2J8-1
    • 10*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    PADI2; Protein-Arginine Deiminase Type-2; Peptidyl Arginine Deiminase 2; PAD-H19; PDI2; PAD2; KIAA0994; CDNA FLJ61278, Highly Similar To Protein-Arginine Deiminase Type-2; Peptidyl Arginine Deiminase, Type II; Peptidylarginine Deiminase II; Protein-Argini

  • AA Sequence

    MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWVEVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDEEGSIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPLSGGRRMTKVVRDFLKAQQVQAPVELYSDWLTVGHVDEFMSFVPIPGTKKFLLLMASTSACYKLFREKQKDGHGEAIMFKGLGGMSSKRITINKILSNESLVQENLYFQRCLDWNRDILKKELGLTEQDIIDLPALFKMDEDHRARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLEMHVRGLLEPLGLECTFIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP

  • Predicted Molecular Mass

    77.1 kDa

  • Molecular Weight

    Approximately 66-85 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5M NaCl, 6% Trehalose, pH 7.4 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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