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PDHA1 Protein, Human (His, Myc)

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Based on 1 Customer Validation

The PDHA1 protein is an important component of the pyruvate dehydrogenase complex and is responsible for coordinating the conversion of pyruvate to acetyl-CoA and CO2. It establishes an important link between glycolysis and the tricarboxylic acid (TCA) cycle, ensuring seamless integration of metabolic pathways. PDHA1 Protein, Human (His, Myc) is the recombinant human-derived PDHA1 protein, expressed by E. coli, with C-Myc and N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PDHA1 Protein, Human (His, Myc)
  • PDHA1 Protein, Human (His, Myc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The PDHA1 protein is an important component of the pyruvate dehydrogenase complex and is responsible for coordinating the conversion of pyruvate to acetyl-CoA and CO2. It establishes an important link between glycolysis and the tricarboxylic acid (TCA) cycle, ensuring seamless integration of metabolic pathways. PDHA1 Protein, Human (His, Myc) is the recombinant human-derived PDHA1 protein, expressed by E. coli, with C-Myc and N-10*His labeled tag.

Background

PDHA1 protein, a crucial constituent of the pyruvate dehydrogenase complex, plays a pivotal role in orchestrating the comprehensive conversion of pyruvate into acetyl-CoA and CO2. This enzymatic process establishes a fundamental link between the glycolytic pathway and the tricarboxylic acid (TCA) cycle, facilitating the seamless integration of metabolic pathways. PDHA1's catalytic function is essential for the efficient transformation of pyruvate, ensuring the flow of carbon substrates into the TCA cycle for subsequent energy generation and metabolic homeostasis within the cell.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-Myc;N-10*His
  • Accession

    P08559-1 (A31-S390)

  • Gene ID
    5160
  • Molecular Construction
    • N-term
    • 10*His
    • (A31-S390)
      Accession # P08559-1
    • Myc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PDHA1; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Testis-Specific Form, Mitochondrial; Prev. PDHA; Pyruvate Dehydrogenase E1 Component Subunit Alpha; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Somatic Form, Mitochondrial; Pyruvate Dehydrog

  • AA Sequence

    ANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

  • Molecular Weight

    Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • PDHA1 Protein, Human (His, Myc) Product Image
    1/16
  • Experimental Validation Results for PDHA1 Protein, Human (His, Myc)
  • PDHA1 Protein, Human (His, Myc) Product Image