PDHA1 Protein, Human (His, Myc)

The PDHA1 protein is an important component of the pyruvate dehydrogenase complex and is responsible for coordinating the conversion of pyruvate to acetyl-CoA and CO2. It establishes an important link between glycolysis and the tricarboxylic acid (TCA) cycle, ensuring seamless integration of metabolic pathways. PDHA1 Protein, Human (His, Myc) is the recombinant human-derived PDHA1 protein, expressed by E. coli, with C-Myc and N-10*His labeled tag.

PDHA1 protein, a crucial constituent of the pyruvate dehydrogenase complex, plays a pivotal role in orchestrating the comprehensive conversion of pyruvate into acetyl-CoA and CO2. This enzymatic process establishes a fundamental link between the glycolytic pathway and the tricarboxylic acid (TCA) cycle, facilitating the seamless integration of metabolic pathways. PDHA1's catalytic function is essential for the efficient transformation of pyruvate, ensuring the flow of carbon substrates into the TCA cycle for subsequent energy generation and metabolic homeostasis within the cell.

Human

E. coli

C-Myc;N-10*His

P08559-1 (A31-S390)

5160

Full Length of Mature Protein

ANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

Approximately 45-50 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.